Generation of a cell line with smooth muscle phenotype from hypertrophied urinary bladder

TY - JOUR

T1 - Generation of a cell line with smooth muscle phenotype from hypertrophied urinary bladder

AU - Zheng, Yongmu

AU - Weber, Wilfried T.

AU - Wang, Shuqin

AU - Wein, Alan J.

AU - Zderic, Stephen A.

AU - Chacko, Samuel

AU - Disanto, Michael E.

PY - 2002

Y1 - 2002

N2 - We have established a cell line from hypertrophied rabbit urinary bladder smooth muscle (SM) that stably expresses SM myosin (SMM). These cells, termed BSM, are spindle shaped and form swirls, similar to the "hills and valleys" described for cultured aortic SM cells. Western blotting revealed that BSM expresses the amino-terminal SMM heavy chain isoform SM-B, the carboxy-terminal SM1 and SM2 isoforms, and SM α-actin. In addition, they express cGMP-dependent protein kinase G, made by contractile SM cells in vitro but not by noncontractile cells synthesizing extracellular matrix. Immunofluorescence studies indicate a homogeneous population of cells expressing α-actin and SMM, including the SM-B isoform, and karyotyping demonstrates a stable 4N chromosomal pattern. These cells also express calcium-dependent myosin light chain kinase and phosphatase activity and contract in response to the muscarinic agonist bethanechol. To our knowledge, BSM is the first visceral SM cell line that expresses the SM-B isoform and might serve as a useful model to study the transcriptional regulation of tissue-specific SMM isoforms in differentiation and pathological SM.

AB - We have established a cell line from hypertrophied rabbit urinary bladder smooth muscle (SM) that stably expresses SM myosin (SMM). These cells, termed BSM, are spindle shaped and form swirls, similar to the "hills and valleys" described for cultured aortic SM cells. Western blotting revealed that BSM expresses the amino-terminal SMM heavy chain isoform SM-B, the carboxy-terminal SM1 and SM2 isoforms, and SM α-actin. In addition, they express cGMP-dependent protein kinase G, made by contractile SM cells in vitro but not by noncontractile cells synthesizing extracellular matrix. Immunofluorescence studies indicate a homogeneous population of cells expressing α-actin and SMM, including the SM-B isoform, and karyotyping demonstrates a stable 4N chromosomal pattern. These cells also express calcium-dependent myosin light chain kinase and phosphatase activity and contract in response to the muscarinic agonist bethanechol. To our knowledge, BSM is the first visceral SM cell line that expresses the SM-B isoform and might serve as a useful model to study the transcriptional regulation of tissue-specific SMM isoforms in differentiation and pathological SM.

UR - https://www.scopus.com/pages/publications/0036088605

UR - https://www.scopus.com/pages/publications/0036088605#tab=citedBy

U2 - 10.1152/ajpcell.00002.2002

DO - 10.1152/ajpcell.00002.2002

M3 - Article

C2 - 12055106

SN - 0363-6143

VL - 283

SP - C373-C382

JO - American Journal of Physiology - Cell Physiology

JF - American Journal of Physiology - Cell Physiology

IS - 1 52-1

ER -