Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation

Chasity P. Janosko; Olivia Shade; Taylor M. Courtney; Trevor J. Horst; Melinda Liu; Sagar D. Khare; Alexander Deiters

doi:10.1021/acsomega.3c03512

Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation

Chasity P. Janosko, Olivia Shade, Taylor M. Courtney, Trevor J. Horst, Melinda Liu, Sagar D. Khare, Alexander Deiters

School of Arts and Sciences, Chemistry & Chemical Biology

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

Abstract

An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the trans-configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure.

Original language	American English
Pages (from-to)	26590-26596
Number of pages	7
Journal	ACS Omega
Volume	8
Issue number	29
DOIs	https://doi.org/10.1021/acsomega.3c03512
State	Published - Jul 25 2023

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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10.1021/acsomega.3c03512

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abstract = "An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the trans-configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure.",

author = "Janosko, {Chasity P.} and Olivia Shade and Courtney, {Taylor M.} and Horst, {Trevor J.} and Melinda Liu and Khare, {Sagar D.} and Alexander Deiters",

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year = "2023",

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AU - Janosko, Chasity P.

AU - Shade, Olivia

AU - Courtney, Taylor M.

AU - Horst, Trevor J.

AU - Liu, Melinda

AU - Khare, Sagar D.

AU - Deiters, Alexander

PY - 2023/7/25

Y1 - 2023/7/25

N2 - An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the trans-configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure.

AB - An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the trans-configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure.

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Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation

Abstract

ASJC Scopus subject areas

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