Genome-wide analysis of Polycomb targets in Drosophila melanogaster

Yuri B. Schwartz, Tatyana G. Kahn, David A. Nix, Xiao Yong Li, Richard Bourgon, Mark Biggin, Vincenzo Pirrotta

Research output: Contribution to journalArticle

424 Citations (Scopus)

Abstract

Polycomb group (PcG) complexes are multiprotein assemblages that bind to chromatin and establish chromatin states leading to epigenetic silencing. PcG proteins regulate homeotic genes in flies and vertebrates, but little is known about other PcG targets and the role of the PcG in development, differentiation and disease. Here, we determined the distribution of the PcG proteins PC, E(Z) and PSC and of trimethylation of histone H3 Lys27 (me3K27) in the D. melanogaster genome. At more than 200 PcG target genes, binding sites for the three PcG proteins colocalize to presumptive Polycomb response elements (PREs). In contrast, H3 me3K27 forms broad domains including the entire transcription unit and regulatory regions. PcG targets are highly enriched in genes encoding transcription factors, but they also include genes coding for receptors, signaling proteins, morphogens and regulators representing all major developmental pathways.

Original languageEnglish (US)
Pages (from-to)700-705
Number of pages6
JournalNature genetics
Volume38
Issue number6
DOIs
StatePublished - Jun 1 2006

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Polycomb-Group Proteins
Drosophila melanogaster
Genome
Chromatin
Genes
Multiprotein Complexes
Homeobox Genes
Nucleic Acid Regulatory Sequences
Response Elements
Epigenomics
Diptera
Histones
Vertebrates
Transcription Factors
Binding Sites
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Schwartz, Y. B., Kahn, T. G., Nix, D. A., Li, X. Y., Bourgon, R., Biggin, M., & Pirrotta, V. (2006). Genome-wide analysis of Polycomb targets in Drosophila melanogaster. Nature genetics, 38(6), 700-705. https://doi.org/10.1038/ng1817
Schwartz, Yuri B. ; Kahn, Tatyana G. ; Nix, David A. ; Li, Xiao Yong ; Bourgon, Richard ; Biggin, Mark ; Pirrotta, Vincenzo. / Genome-wide analysis of Polycomb targets in Drosophila melanogaster. In: Nature genetics. 2006 ; Vol. 38, No. 6. pp. 700-705.
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Schwartz, YB, Kahn, TG, Nix, DA, Li, XY, Bourgon, R, Biggin, M & Pirrotta, V 2006, 'Genome-wide analysis of Polycomb targets in Drosophila melanogaster', Nature genetics, vol. 38, no. 6, pp. 700-705. https://doi.org/10.1038/ng1817

Genome-wide analysis of Polycomb targets in Drosophila melanogaster. / Schwartz, Yuri B.; Kahn, Tatyana G.; Nix, David A.; Li, Xiao Yong; Bourgon, Richard; Biggin, Mark; Pirrotta, Vincenzo.

In: Nature genetics, Vol. 38, No. 6, 01.06.2006, p. 700-705.

Research output: Contribution to journalArticle

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Schwartz YB, Kahn TG, Nix DA, Li XY, Bourgon R, Biggin M et al. Genome-wide analysis of Polycomb targets in Drosophila melanogaster. Nature genetics. 2006 Jun 1;38(6):700-705. https://doi.org/10.1038/ng1817