Genomic organization and promoter analysis of the gene ifngr2 encoding the second chain of the mouse interferon-γ receptor

C. Ebensperger, S. Rhee, G. Muthukumaran, D. Lembo, R. Donnelly, S. Pestka, Z. Dembic

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A clone containing the gene ifngr2 for the second chain (IFN-γR2) of the mouse interferon γ receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5'-flanking region several transcription initiation sites between 27 and 136 nucleotides upstream from the translation initiation codon were mapped. This region has a high GC content, but no TATA or CAAT box. Potential binding sites were found for transcription factors Sp1, AP-2, NF1, EGR and NFκB. Promoter activity was assayed with a series of constructs with firefly luciferase as a reporter gene, under the control of the promoter fragments of various lengths. This region showed promoter activity in transiently transfected Chinese hamster ovary cells.

Original languageEnglish (US)
Pages (from-to)599-606
Number of pages8
JournalScandinavian Journal of Immunology
Volume44
Issue number6
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Immunology

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