Abstract
We present a novel approach to control endothelial tubulogenesis by spatially patterning cells within micromolded collagen gels. Endothelial cells cultured within microscale channels that were filled with collagen gel organized into tubes with lumens within 24-48h of seeding. These tubes extended up to 1cm in length, and exhibited cell-cell junction formation characteristic of early stage capillary vessels. Tube diameter could be controlled by varying collagen concentrations or channel width. The geometry of the microfabricated template also could be used to guide the development of branches during tube formation, allowing for the generation of more complex capillary architectures. Time-lapse imaging of tube formation revealed a highly dynamic process involving coalescence of endothelial cells, reorganization and alignment of collagen fibers into a central core, and arrangement of cells into cords. This platform may be of use to generate geometrically defined vascular networks for tissue engineering applications as well as a means to better understand the process of endothelial tubulogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2255-2263 |
| Number of pages | 9 |
| Journal | Tissue Engineering - Part A |
| Volume | 16 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2010 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Medicine(all)
- Biochemistry
- Biomedical Engineering
- Biomaterials
Cite this
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Geometrically controlled endothelial tubulogenesis in micropatterned gels. / Raghavan, Srivatsan; Nelson, Celeste M.; Baranski, Jan D.; Lim, Emerson; Chen, Christopher S.
In: Tissue Engineering - Part A, Vol. 16, No. 7, 01.07.2010, p. 2255-2263.Research output: Contribution to journal › Article
TY - JOUR
T1 - Geometrically controlled endothelial tubulogenesis in micropatterned gels
AU - Raghavan, Srivatsan
AU - Nelson, Celeste M.
AU - Baranski, Jan D.
AU - Lim, Emerson
AU - Chen, Christopher S.
PY - 2010/7/1
Y1 - 2010/7/1
N2 - We present a novel approach to control endothelial tubulogenesis by spatially patterning cells within micromolded collagen gels. Endothelial cells cultured within microscale channels that were filled with collagen gel organized into tubes with lumens within 24-48h of seeding. These tubes extended up to 1cm in length, and exhibited cell-cell junction formation characteristic of early stage capillary vessels. Tube diameter could be controlled by varying collagen concentrations or channel width. The geometry of the microfabricated template also could be used to guide the development of branches during tube formation, allowing for the generation of more complex capillary architectures. Time-lapse imaging of tube formation revealed a highly dynamic process involving coalescence of endothelial cells, reorganization and alignment of collagen fibers into a central core, and arrangement of cells into cords. This platform may be of use to generate geometrically defined vascular networks for tissue engineering applications as well as a means to better understand the process of endothelial tubulogenesis.
AB - We present a novel approach to control endothelial tubulogenesis by spatially patterning cells within micromolded collagen gels. Endothelial cells cultured within microscale channels that were filled with collagen gel organized into tubes with lumens within 24-48h of seeding. These tubes extended up to 1cm in length, and exhibited cell-cell junction formation characteristic of early stage capillary vessels. Tube diameter could be controlled by varying collagen concentrations or channel width. The geometry of the microfabricated template also could be used to guide the development of branches during tube formation, allowing for the generation of more complex capillary architectures. Time-lapse imaging of tube formation revealed a highly dynamic process involving coalescence of endothelial cells, reorganization and alignment of collagen fibers into a central core, and arrangement of cells into cords. This platform may be of use to generate geometrically defined vascular networks for tissue engineering applications as well as a means to better understand the process of endothelial tubulogenesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=77954469767&partnerID=8YFLogxK
U2 - https://doi.org/10.1089/ten.tea.2009.0584
DO - https://doi.org/10.1089/ten.tea.2009.0584
M3 - Article
C2 - 20180698
VL - 16
SP - 2255
EP - 2263
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
SN - 1937-3341
IS - 7
ER -