Glutamate carboxypeptidase II is expressed by astrocytes in the adult rat nervous system

Urs V. Berger; Ruth Luthi-Carter; Lucius A. Passani; Stella Elkabes; Ira Black; Christine Konradi; Joseph T. Coyle

doi:10.1002/(SICI)1096-9861(19991206)415:1

Glutamate carboxypeptidase II is expressed by astrocytes in the adult rat nervous system

Urs V. Berger, Ruth Luthi-Carter, Lucius A. Passani, Stella Elkabes, Ira Black, Christine Konradi, Joseph T. Coyle

New Jersey Medical School (NJMS), Neurosurgery

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

The enzyme glutamate carboxypeptidase II (GCP II) has been cloned from rat brain and human prostate. This enzyme, which catabolizes the neuropeptide N-acetylaspartylglutamate, has also been known as N-acetylated α-linked acidic dipeptidase (NAALADase), and is identical to the prostate-specific membrane antigen and to the jejunal folylpoly-γ-glutamate carboxypeptidase. The goals of the present study were to elucidate the cell specificity and regional pattern of GCP II expression in the rat nervous system by using Northern blots and enzymatic assays of brain and subfractionated primary neuronal and glial cultures together with in situ hybridization histochemistry (ISHH) in sections of adult rat tissue. GCP II activity was assayed in astrocyte cultures (4.4 pmol/mg protein per minute), neuronal- glial cocultures (2.5 pmol/mg protein per minute) and neuron-enriched cultures (0.38 pmol/mg protein per minute), with the activity in each preparation correlating to its astrocytic content (r = 0.99). No activity was detected in cultured oligodendrocytes or microglia. Northern blots probed with a GCP II cDNA detected mRNAs exclusively in activity-positive cell preparations. ISHH results show that GCP II is expressed by virtually all astrocytes, by Bergmann glial cells in cerebellum, by Muller cells in retina and by the satellite cells in dorsal root ganglia. Astrocytes in select groups of nuclei (e.g., habenula, supraoptic nucleus, pontine nucleus) contained pronounced levels of GCP II message. The data of the present study suggest that GCP II is expressed in the adult rat nervous system exclusively in astrocytic glial cells.

Original language	American English
Pages (from-to)	52-64
Number of pages	13
Journal	Journal of Comparative Neurology
Volume	415
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1096-9861(19991206)415:1
State	Published - Dec 6 1999

ASJC Scopus subject areas

General Neuroscience

Keywords

Folylpoly-γ-glutamate carboxypeptidase
Gliosis
Glutamate carboxypeptidase II
In situ hybridization
NAALADase
Prostate-specific membrane antigen

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10.1002/(SICI)1096-9861(19991206)415:1

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title = "Glutamate carboxypeptidase II is expressed by astrocytes in the adult rat nervous system",

abstract = "The enzyme glutamate carboxypeptidase II (GCP II) has been cloned from rat brain and human prostate. This enzyme, which catabolizes the neuropeptide N-acetylaspartylglutamate, has also been known as N-acetylated α-linked acidic dipeptidase (NAALADase), and is identical to the prostate-specific membrane antigen and to the jejunal folylpoly-γ-glutamate carboxypeptidase. The goals of the present study were to elucidate the cell specificity and regional pattern of GCP II expression in the rat nervous system by using Northern blots and enzymatic assays of brain and subfractionated primary neuronal and glial cultures together with in situ hybridization histochemistry (ISHH) in sections of adult rat tissue. GCP II activity was assayed in astrocyte cultures (4.4 pmol/mg protein per minute), neuronal- glial cocultures (2.5 pmol/mg protein per minute) and neuron-enriched cultures (0.38 pmol/mg protein per minute), with the activity in each preparation correlating to its astrocytic content (r = 0.99). No activity was detected in cultured oligodendrocytes or microglia. Northern blots probed with a GCP II cDNA detected mRNAs exclusively in activity-positive cell preparations. ISHH results show that GCP II is expressed by virtually all astrocytes, by Bergmann glial cells in cerebellum, by Muller cells in retina and by the satellite cells in dorsal root ganglia. Astrocytes in select groups of nuclei (e.g., habenula, supraoptic nucleus, pontine nucleus) contained pronounced levels of GCP II message. The data of the present study suggest that GCP II is expressed in the adult rat nervous system exclusively in astrocytic glial cells.",

keywords = "Folylpoly-γ-glutamate carboxypeptidase, Gliosis, Glutamate carboxypeptidase II, In situ hybridization, NAALADase, Prostate-specific membrane antigen",

author = "Berger, {Urs V.} and Ruth Luthi-Carter and Passani, {Lucius A.} and Stella Elkabes and Ira Black and Christine Konradi and Coyle, {Joseph T.}",

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T1 - Glutamate carboxypeptidase II is expressed by astrocytes in the adult rat nervous system

AU - Berger, Urs V.

AU - Luthi-Carter, Ruth

AU - Passani, Lucius A.

AU - Elkabes, Stella

AU - Black, Ira

AU - Konradi, Christine

AU - Coyle, Joseph T.

PY - 1999/12/6

Y1 - 1999/12/6

N2 - The enzyme glutamate carboxypeptidase II (GCP II) has been cloned from rat brain and human prostate. This enzyme, which catabolizes the neuropeptide N-acetylaspartylglutamate, has also been known as N-acetylated α-linked acidic dipeptidase (NAALADase), and is identical to the prostate-specific membrane antigen and to the jejunal folylpoly-γ-glutamate carboxypeptidase. The goals of the present study were to elucidate the cell specificity and regional pattern of GCP II expression in the rat nervous system by using Northern blots and enzymatic assays of brain and subfractionated primary neuronal and glial cultures together with in situ hybridization histochemistry (ISHH) in sections of adult rat tissue. GCP II activity was assayed in astrocyte cultures (4.4 pmol/mg protein per minute), neuronal- glial cocultures (2.5 pmol/mg protein per minute) and neuron-enriched cultures (0.38 pmol/mg protein per minute), with the activity in each preparation correlating to its astrocytic content (r = 0.99). No activity was detected in cultured oligodendrocytes or microglia. Northern blots probed with a GCP II cDNA detected mRNAs exclusively in activity-positive cell preparations. ISHH results show that GCP II is expressed by virtually all astrocytes, by Bergmann glial cells in cerebellum, by Muller cells in retina and by the satellite cells in dorsal root ganglia. Astrocytes in select groups of nuclei (e.g., habenula, supraoptic nucleus, pontine nucleus) contained pronounced levels of GCP II message. The data of the present study suggest that GCP II is expressed in the adult rat nervous system exclusively in astrocytic glial cells.

AB - The enzyme glutamate carboxypeptidase II (GCP II) has been cloned from rat brain and human prostate. This enzyme, which catabolizes the neuropeptide N-acetylaspartylglutamate, has also been known as N-acetylated α-linked acidic dipeptidase (NAALADase), and is identical to the prostate-specific membrane antigen and to the jejunal folylpoly-γ-glutamate carboxypeptidase. The goals of the present study were to elucidate the cell specificity and regional pattern of GCP II expression in the rat nervous system by using Northern blots and enzymatic assays of brain and subfractionated primary neuronal and glial cultures together with in situ hybridization histochemistry (ISHH) in sections of adult rat tissue. GCP II activity was assayed in astrocyte cultures (4.4 pmol/mg protein per minute), neuronal- glial cocultures (2.5 pmol/mg protein per minute) and neuron-enriched cultures (0.38 pmol/mg protein per minute), with the activity in each preparation correlating to its astrocytic content (r = 0.99). No activity was detected in cultured oligodendrocytes or microglia. Northern blots probed with a GCP II cDNA detected mRNAs exclusively in activity-positive cell preparations. ISHH results show that GCP II is expressed by virtually all astrocytes, by Bergmann glial cells in cerebellum, by Muller cells in retina and by the satellite cells in dorsal root ganglia. Astrocytes in select groups of nuclei (e.g., habenula, supraoptic nucleus, pontine nucleus) contained pronounced levels of GCP II message. The data of the present study suggest that GCP II is expressed in the adult rat nervous system exclusively in astrocytic glial cells.

KW - Folylpoly-γ-glutamate carboxypeptidase

KW - Gliosis

KW - Glutamate carboxypeptidase II

KW - In situ hybridization

KW - NAALADase

KW - Prostate-specific membrane antigen

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Glutamate carboxypeptidase II is expressed by astrocytes in the adult rat nervous system

Abstract

ASJC Scopus subject areas

Keywords

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