Abstract
Graft-versus-host disease (GvHD) is a potentially devastating complication of allogeneic hematopoietic cell transplantation (allo-HCT). The use of ex vivo T-cell depletion (TCD) of hematopoietic cell grafts has significantly reduced the risk of GvHD. The recognition that GvHD is mediated by donor-derived T-cells led to preclinical and clinical exploration of TCD as a strategy to reduce the risk of GvHD. TCD of the graft can be performed by either positive or negative selection of CD34+ cells. Negative selection by extraction of donor lymphocytes has been achieved through physical methods such as soybean lectin agglutination (SBA) followed by sheep red blood cell (sRBC)-rosette depletion (E-rosetting), or counterflow elution. At present, positive selection by extraction of CD34+ cells from the graft is the most common technique in clinical use to achieve TCD of the graft. The use of ex vivo TCD grafts has significantly reduced the risk of GvHD in a variety of hematologic malignancies.
| Original language | English |
|---|---|
| Title of host publication | Clinical Manual of Blood and Bone Marrow Transplantation |
| Publisher | wiley |
| Pages | 66-72 |
| Number of pages | 7 |
| ISBN (Electronic) | 9781119095491 |
| ISBN (Print) | 9781119095453 |
| DOIs | |
| State | Published - Jan 1 2017 |
ASJC Scopus subject areas
- General Medicine
Keywords
- Allogeneic hematopoietic cell transplantation
- Graft-versus-host disease
- Hematologic malignancies
- Hematopoietic cell grafts
- Negative selection
- Optimal patient selection
- Positive selection
- Soybean lectin agglutination
- T-cell depletion