Heat shock causes destabilization of specific mRNAs and destruction of endoplasmic reticulum in barley aleurone cells

TY - JOUR

T1 - Heat shock causes destabilization of specific mRNAs and destruction of endoplasmic reticulum in barley aleurone cells

AU - Belanger, F. C.

AU - Brodl, M. R.

AU - Ho, T. H.D.

PY - 1986

Y1 - 1986

N2 - In response to a phytohormone, gibberellic acid, the aleurone layers of barley seeds synthesize and secrete α-amylases, which are coded by a set of stable mRNAs. When aleurone layers are subjected to heat shock treatment, the synthesis of α-amylase is suppressed while heat shock proteins are induced. The suppression of α-amylase synthesis is not the result of translational control as reported in several other systems. Rather, the sequences of α-amylase mRNA are rapidly degraded during heat shock as shown by in vitro translation and dot blot hybridization, with a cDNA probe. Upon recovery from heat shock, the tissue resumes the synthesis of α-amylase in 2-4 hr. However, in the presence of a transcription inhibitor, cordycepin, the resumption of synthesis of α-amylase does not take place, indicating that new transcription of α-amylase genes is necessary for this recovery process. The degradation of α-amylase mRNAs correlates with the rapid destruction of endoplasmic reticulum as observed by electron microscopy, a phenomenon that has not been reported previously as a heat shock response. Since α-amylase mRNA is associated with the endoplasmic reticulum via membrane-bound polyribosomes, we suggest that the destruction of the endoplasmic reticulum during heat shock, causes the destabilization and the eventual degradation of α-amylase mRNA.

AB - In response to a phytohormone, gibberellic acid, the aleurone layers of barley seeds synthesize and secrete α-amylases, which are coded by a set of stable mRNAs. When aleurone layers are subjected to heat shock treatment, the synthesis of α-amylase is suppressed while heat shock proteins are induced. The suppression of α-amylase synthesis is not the result of translational control as reported in several other systems. Rather, the sequences of α-amylase mRNA are rapidly degraded during heat shock as shown by in vitro translation and dot blot hybridization, with a cDNA probe. Upon recovery from heat shock, the tissue resumes the synthesis of α-amylase in 2-4 hr. However, in the presence of a transcription inhibitor, cordycepin, the resumption of synthesis of α-amylase does not take place, indicating that new transcription of α-amylase genes is necessary for this recovery process. The degradation of α-amylase mRNAs correlates with the rapid destruction of endoplasmic reticulum as observed by electron microscopy, a phenomenon that has not been reported previously as a heat shock response. Since α-amylase mRNA is associated with the endoplasmic reticulum via membrane-bound polyribosomes, we suggest that the destruction of the endoplasmic reticulum during heat shock, causes the destabilization and the eventual degradation of α-amylase mRNA.

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U2 - 10.1073/pnas.83.5.1354

DO - 10.1073/pnas.83.5.1354

M3 - Article

C2 - 3485284

SN - 0027-8424

VL - 83

SP - 1354

EP - 1358

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 5

ER -