Hematopoietic Stem Cell Collections and Cellular Therapies

TY - CHAP

T1 - Hematopoietic Stem Cell Collections and Cellular Therapies

AU - Vasovic, Ljiljana V.

AU - Reich-Slotky, Ronit

AU - Goel, Ruchika

N1 - Publisher Copyright: © 2018 Elsevier Inc. All rights reserved.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The advancement of hematopoietic progenitor cell (HPC) transplant has increased the chance of cure for a number of hematologic malignancies and other nonmalignant hematologic and congenital diseases. HPCs can be collected from a patient, stored, and transplanted back to the patient after chemotherapy/radiotherapy, as an autologous HPC transplant (HPCT). Infusion of HPCs from siblings or matched related donors or volunteer matched unrelated donors constitutes allogeneic HPCT. Finding an optimal donor for allogeneic HPCT by extensive human leukocyte antigen (HLA) matching between donor and recipient is the most important factor influencing transplant outcomes. The traditional source of HPCs is bone marrow; however, peripheral blood stem cells, mobilized to circulation and harvested by apheresis, have become the preferred graft source because of procurement of higher dosage and convenience of collection. If a full HLA match is not available, umbilical cord blood cells can be used, either as a single graft or in combination with other cellular therapy products (CTPs). A brief overview of donor matching and selection, choice of graft source, and CTP collection procedure are discussed. Laboratory graft characterization and processing, such as HPC CD34+ selection, CTP cryopreservation, storage, thawing, infusion, and impact of those parameters on transplant success and transplant-related outcomes, are reviewed. HPC infusion reactions and posttransplant monitoring including engraftment are outlined. New investigational products reaching clinical practice, such as expanded or genetically modified stem cells, immunotherapy, and regenerative CTP, are also discussed.

AB - The advancement of hematopoietic progenitor cell (HPC) transplant has increased the chance of cure for a number of hematologic malignancies and other nonmalignant hematologic and congenital diseases. HPCs can be collected from a patient, stored, and transplanted back to the patient after chemotherapy/radiotherapy, as an autologous HPC transplant (HPCT). Infusion of HPCs from siblings or matched related donors or volunteer matched unrelated donors constitutes allogeneic HPCT. Finding an optimal donor for allogeneic HPCT by extensive human leukocyte antigen (HLA) matching between donor and recipient is the most important factor influencing transplant outcomes. The traditional source of HPCs is bone marrow; however, peripheral blood stem cells, mobilized to circulation and harvested by apheresis, have become the preferred graft source because of procurement of higher dosage and convenience of collection. If a full HLA match is not available, umbilical cord blood cells can be used, either as a single graft or in combination with other cellular therapy products (CTPs). A brief overview of donor matching and selection, choice of graft source, and CTP collection procedure are discussed. Laboratory graft characterization and processing, such as HPC CD34+ selection, CTP cryopreservation, storage, thawing, infusion, and impact of those parameters on transplant success and transplant-related outcomes, are reviewed. HPC infusion reactions and posttransplant monitoring including engraftment are outlined. New investigational products reaching clinical practice, such as expanded or genetically modified stem cells, immunotherapy, and regenerative CTP, are also discussed.

KW - Bone marrow transplantation

KW - Cellular therapy processing

KW - Cellular therapy products

KW - Hematopoietic progenitor cell transplantation

KW - Stem cell donor

KW - Stem cells collection

UR - https://www.scopus.com/pages/publications/85054375120

U2 - 10.1016/B978-0-323-54458-0.00013-1

DO - 10.1016/B978-0-323-54458-0.00013-1

M3 - Chapter

SN - 9780323544597

SP - 151

EP - 167

BT - Clinical Principles of Transfusion Medicine

PB - Elsevier

ER -