High affinity binding of transferrin in cultures of embryonic neurons from the chick retina

TY - JOUR

T1 - High affinity binding of transferrin in cultures of embryonic neurons from the chick retina

AU - Hyndman, Arnold G.

PY - 1991/11/8

Y1 - 1991/11/8

N2 - Immunohistochemical and autoradiographic analysis of neuronal cultures from embryonic day 8 (E8) and day 11 (E11) chick retina indicate that transferrin receptors and binding sites are present on soma and neurites. Cultures maintained in the presence of transferrin expressed elevated transferrin binding due to an increase in the number of transferrin receptors. Cultures from E11 neural retina exhibited a decrease in transferrin binding when compared to E8 cultures. This appears to be due to a decrease in the number of binding sites. Neurons maintained in a transferrin-free medium supplemented with 0.4 μM of iron sulfate generally expressed slight increases in transferrin binding.

AB - Immunohistochemical and autoradiographic analysis of neuronal cultures from embryonic day 8 (E8) and day 11 (E11) chick retina indicate that transferrin receptors and binding sites are present on soma and neurites. Cultures maintained in the presence of transferrin expressed elevated transferrin binding due to an increase in the number of transferrin receptors. Cultures from E11 neural retina exhibited a decrease in transferrin binding when compared to E8 cultures. This appears to be due to a decrease in the number of binding sites. Neurons maintained in a transferrin-free medium supplemented with 0.4 μM of iron sulfate generally expressed slight increases in transferrin binding.

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U2 - https://doi.org/10.1016/0006-8993(91)91361-4

DO - https://doi.org/10.1016/0006-8993(91)91361-4

M3 - Article

C2 - 1777815

SN - 0006-8993

VL - 564

SP - 127

EP - 131

JO - Brain research

JF - Brain research

IS - 1

ER -