High-level conjugation of chelating agents onto immunoglobulins: use of an intermediary poly(l-lysine)-diethylenetriaminepentaacetic acid carrier

TY - JOUR

T1 - High-level conjugation of chelating agents onto immunoglobulins

T2 - use of an intermediary poly(l-lysine)-diethylenetriaminepentaacetic acid carrier

AU - Manabe, Yuichi

AU - Longley, Clifford

AU - Furmanski, Philip

PY - 1986/10/1

Y1 - 1986/10/1

N2 - Diethylenetriaminepentaacetic acid (DTPA), a strong chelating agent, was covalently linked to murine monoclonal anti-HLA IgG1 antibody (H-1) with the use of poly(l-lysine) (Mr 14 000) as a multivalent, intermediary carrier, via thiol-disulfide exchange reaction. The conjugates contained up to 42.5 mol DTPA per mol antibody, and retained over 90% of their antibody activity in vitro. The conjugates incorporated gadolinium (Gd) through an exchange reaction with Gd-EDTA, used to prevent colloid formation and nonspecific binding of the free metal. The IgG-poly(l-lysine)-DTPA-Gd had a greater effect per mol on proton relaxation rates than DTPA-Gd itself. Use of poly(l-lysine) as an intermediary carrier for attachment of chelating agents to IgG thus offers great potential for achieving high-specific-activity conjugates, particularly for use as biologically specific contrast agents in nuclear magnetic resonance imaging.

AB - Diethylenetriaminepentaacetic acid (DTPA), a strong chelating agent, was covalently linked to murine monoclonal anti-HLA IgG1 antibody (H-1) with the use of poly(l-lysine) (Mr 14 000) as a multivalent, intermediary carrier, via thiol-disulfide exchange reaction. The conjugates contained up to 42.5 mol DTPA per mol antibody, and retained over 90% of their antibody activity in vitro. The conjugates incorporated gadolinium (Gd) through an exchange reaction with Gd-EDTA, used to prevent colloid formation and nonspecific binding of the free metal. The IgG-poly(l-lysine)-DTPA-Gd had a greater effect per mol on proton relaxation rates than DTPA-Gd itself. Use of poly(l-lysine) as an intermediary carrier for attachment of chelating agents to IgG thus offers great potential for achieving high-specific-activity conjugates, particularly for use as biologically specific contrast agents in nuclear magnetic resonance imaging.

KW - Diethylenetriaminepentaacetic acid

KW - Gadolinium

KW - Immunoglobulin conjugate

KW - Monoclonal antibody

KW - Poly(l-lysine)

UR - https://www.scopus.com/pages/publications/0022466862

UR - https://www.scopus.com/pages/publications/0022466862#tab=citedBy

U2 - 10.1016/0304-4165(86)90285-0

DO - 10.1016/0304-4165(86)90285-0

M3 - Article

C2 - 3756213

SN - 0304-4165

VL - 883

SP - 460

EP - 467

JO - BBA - General Subjects

JF - BBA - General Subjects

IS - 3

ER -