Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination

Pratik Datta; Rahul Ukey; Natalie Bruiners; William Honnen; Mary Carayannopoulos; Charles Reichman; Alok Choudhary; Alberta Onyuka; Deborah Handler; Valentina Guerrini; Pankaj K. Mishra; Hannah K. Dewald; Alfred Lardizabal; Leeba Lederer; Aliza Leiser; Sabiha Hussain; Sugeet K. Jagpal; Jared Radbel; Tanaya Bhowmick; Daniel B. Horton; Emily S. Barrett; Yingda L. Xie; Patricia Fitzgerald-Bocarsly; Stanley H. Weiss; Melissa Woortman; Heta Parmar; Jason Roy; Maria Gloria Dominguez-Bello; Martin J. Blaser; Jeffrey L. Carson; Reynold A. Panettieri; Steven K. Libutti; Henry F. Raymond; Abraham Pinter; Maria Laura Gennaro

doi:10.1016/j.jim.2021.113165

Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination

Pratik Datta, Rahul Ukey, Natalie Bruiners, William Honnen, Mary Carayannopoulos, Charles Reichman, Alok Choudhary, Alberta Onyuka, Deborah Handler, Valentina Guerrini, Pankaj K. Mishra, Hannah K. Dewald, Alfred Lardizabal, Leeba Lederer, Aliza Leiser, Sabiha Hussain, Sugeet K. Jagpal, Jared Radbel, Tanaya Bhowmick, Daniel B. HortonEmily S. Barrett, Yingda L. Xie, Patricia Fitzgerald-Bocarsly, Stanley H. Weiss, Melissa Woortman, Heta Parmar, Jason Roy, Maria Gloria Dominguez-Bello, Martin J. Blaser, Jeffrey L. Carson, Reynold A. Panettieri, Steven K. Libutti, Henry F. Raymond, Abraham Pinter, Maria Laura Gennaro

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

Abstract

Monitoring the burden and spread of infection with the new coronavirus SARS-CoV-2, whether within small communities or in large geographical settings, is of paramount importance for public health purposes. Serology, which detects the host antibody response to the infection, is the most appropriate tool for this task, since virus-derived markers are most reliably detected during the acute phase of infection. Here we show that our ELISA protocol, which is based on antibody binding to the Receptor Binding Domain (RBD) of the S1 subunit of the viral Spike protein expressed as a novel fusion protein, detects antibody responses to SARS-CoV-2 infection and vaccination. We also show that our ELISA is accurate and versatile. It compares favorably with commercial assays widely used in clinical practice to determine exposure to SARS-CoV-2. Moreover, our protocol accommodates use of various blood- and non-blood-derived biospecimens, such as breast milk, as well as dried blood obtained with microsampling cartridges that are appropriate for remote collection. As a result, our RBD-based ELISA protocols are well suited for seroepidemiology and other large-scale studies requiring parsimonious sample collection outside of healthcare settings.

Original language	American English
Article number	113165
Journal	Journal of Immunological Methods
Volume	499
DOIs	https://doi.org/10.1016/j.jim.2021.113165
State	Published - Dec 2021

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Keywords

Breast milk
COVID-19
Microsampling
Seroepidemiology

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10.1016/j.jim.2021.113165

Cite this

Datta, P., Ukey, R., Bruiners, N., Honnen, W., Carayannopoulos, M., Reichman, C., Choudhary, A., Onyuka, A., Handler, D., Guerrini, V., Mishra, P. K., Dewald, H. K., Lardizabal, A., Lederer, L., Leiser, A., Hussain, S., Jagpal, S. K., Radbel, J., Bhowmick, T., ... Gennaro, M. L. (2021). Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination. Journal of Immunological Methods, 499, Article 113165. https://doi.org/10.1016/j.jim.2021.113165

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title = "Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination",

abstract = "Monitoring the burden and spread of infection with the new coronavirus SARS-CoV-2, whether within small communities or in large geographical settings, is of paramount importance for public health purposes. Serology, which detects the host antibody response to the infection, is the most appropriate tool for this task, since virus-derived markers are most reliably detected during the acute phase of infection. Here we show that our ELISA protocol, which is based on antibody binding to the Receptor Binding Domain (RBD) of the S1 subunit of the viral Spike protein expressed as a novel fusion protein, detects antibody responses to SARS-CoV-2 infection and vaccination. We also show that our ELISA is accurate and versatile. It compares favorably with commercial assays widely used in clinical practice to determine exposure to SARS-CoV-2. Moreover, our protocol accommodates use of various blood- and non-blood-derived biospecimens, such as breast milk, as well as dried blood obtained with microsampling cartridges that are appropriate for remote collection. As a result, our RBD-based ELISA protocols are well suited for seroepidemiology and other large-scale studies requiring parsimonious sample collection outside of healthcare settings.",

keywords = "Breast milk, COVID-19, Microsampling, Seroepidemiology",

author = "Pratik Datta and Rahul Ukey and Natalie Bruiners and William Honnen and Mary Carayannopoulos and Charles Reichman and Alok Choudhary and Alberta Onyuka and Deborah Handler and Valentina Guerrini and Mishra, {Pankaj K.} and Dewald, {Hannah K.} and Alfred Lardizabal and Leeba Lederer and Aliza Leiser and Sabiha Hussain and Jagpal, {Sugeet K.} and Jared Radbel and Tanaya Bhowmick and Horton, {Daniel B.} and Barrett, {Emily S.} and Xie, {Yingda L.} and Patricia Fitzgerald-Bocarsly and Weiss, {Stanley H.} and Melissa Woortman and Heta Parmar and Jason Roy and Dominguez-Bello, {Maria Gloria} and Blaser, {Martin J.} and Carson, {Jeffrey L.} and Panettieri, {Reynold A.} and Libutti, {Steven K.} and Raymond, {Henry F.} and Abraham Pinter and Gennaro, {Maria Laura}",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = dec,

doi = "10.1016/j.jim.2021.113165",

language = "American English",

volume = "499",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

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Datta, P, Ukey, R, Bruiners, N, Honnen, W, Carayannopoulos, M, Reichman, C, Choudhary, A, Onyuka, A, Handler, D, Guerrini, V, Mishra, PK, Dewald, HK, Lardizabal, A, Lederer, L, Leiser, A , Hussain, S , Jagpal, SK , Radbel, J , Bhowmick, T , Horton, DB , Barrett, ES , Xie, YL , Fitzgerald-Bocarsly, P , Weiss, SH, Woortman, M, Parmar, H, Roy, J , Dominguez-Bello, MG , Blaser, MJ , Carson, JL , Panettieri, RA , Libutti, SK , Raymond, HF , Pinter, A & Gennaro, ML 2021, 'Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination', Journal of Immunological Methods, vol. 499, 113165. https://doi.org/10.1016/j.jim.2021.113165

TY - JOUR

T1 - Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination

AU - Datta, Pratik

AU - Ukey, Rahul

AU - Bruiners, Natalie

AU - Honnen, William

AU - Carayannopoulos, Mary

AU - Reichman, Charles

AU - Choudhary, Alok

AU - Onyuka, Alberta

AU - Handler, Deborah

AU - Guerrini, Valentina

AU - Mishra, Pankaj K.

AU - Dewald, Hannah K.

AU - Lardizabal, Alfred

AU - Lederer, Leeba

AU - Leiser, Aliza

AU - Hussain, Sabiha

AU - Jagpal, Sugeet K.

AU - Radbel, Jared

AU - Bhowmick, Tanaya

AU - Horton, Daniel B.

AU - Barrett, Emily S.

AU - Xie, Yingda L.

AU - Fitzgerald-Bocarsly, Patricia

AU - Weiss, Stanley H.

AU - Woortman, Melissa

AU - Parmar, Heta

AU - Roy, Jason

AU - Dominguez-Bello, Maria Gloria

AU - Blaser, Martin J.

AU - Carson, Jeffrey L.

AU - Panettieri, Reynold A.

AU - Libutti, Steven K.

AU - Raymond, Henry F.

AU - Pinter, Abraham

AU - Gennaro, Maria Laura

PY - 2021/12

Y1 - 2021/12

N2 - Monitoring the burden and spread of infection with the new coronavirus SARS-CoV-2, whether within small communities or in large geographical settings, is of paramount importance for public health purposes. Serology, which detects the host antibody response to the infection, is the most appropriate tool for this task, since virus-derived markers are most reliably detected during the acute phase of infection. Here we show that our ELISA protocol, which is based on antibody binding to the Receptor Binding Domain (RBD) of the S1 subunit of the viral Spike protein expressed as a novel fusion protein, detects antibody responses to SARS-CoV-2 infection and vaccination. We also show that our ELISA is accurate and versatile. It compares favorably with commercial assays widely used in clinical practice to determine exposure to SARS-CoV-2. Moreover, our protocol accommodates use of various blood- and non-blood-derived biospecimens, such as breast milk, as well as dried blood obtained with microsampling cartridges that are appropriate for remote collection. As a result, our RBD-based ELISA protocols are well suited for seroepidemiology and other large-scale studies requiring parsimonious sample collection outside of healthcare settings.

AB - Monitoring the burden and spread of infection with the new coronavirus SARS-CoV-2, whether within small communities or in large geographical settings, is of paramount importance for public health purposes. Serology, which detects the host antibody response to the infection, is the most appropriate tool for this task, since virus-derived markers are most reliably detected during the acute phase of infection. Here we show that our ELISA protocol, which is based on antibody binding to the Receptor Binding Domain (RBD) of the S1 subunit of the viral Spike protein expressed as a novel fusion protein, detects antibody responses to SARS-CoV-2 infection and vaccination. We also show that our ELISA is accurate and versatile. It compares favorably with commercial assays widely used in clinical practice to determine exposure to SARS-CoV-2. Moreover, our protocol accommodates use of various blood- and non-blood-derived biospecimens, such as breast milk, as well as dried blood obtained with microsampling cartridges that are appropriate for remote collection. As a result, our RBD-based ELISA protocols are well suited for seroepidemiology and other large-scale studies requiring parsimonious sample collection outside of healthcare settings.

KW - Breast milk

KW - COVID-19

KW - Microsampling

KW - Seroepidemiology

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UR - http://www.scopus.com/inward/citedby.url?scp=85118329598&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2021.113165

DO - 10.1016/j.jim.2021.113165

M3 - Article

C2 - 34634317

SN - 0022-1759

VL - 499

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

M1 - 113165

ER -

Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination

Abstract

ASJC Scopus subject areas

Keywords

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