Histochemical Alterations in One Lung Ventilation

Kingsley Yin, Elizabeth Gribbin, Steven Emanuel, Rebecca Orndorff, Jean Walker, James Weese, Manucher Fallahnejad

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: One lung ventilation is a commonly performed surgical procedure. Although there have been several reports showing that one-lung ventilation can cause pathophysiological alterations such as pulmonary hypoxic vasoconstriction and intrapulmonary shunting, there have been virtually no reports on the effects of one-lung ventilation on lung histology. Materials and methods: Yorkshire pigs (11-17 kg) were anesthetized, a tracheotomy performed and a tracheal tube inserted. The chest was opened and one lung ventilation (OLV), was induced by clamping of the right main bronchus. OLV was continued for 60 min before the clamp was removed and two lung ventilation (TLV) started. TLV was continued for 30 to 60 min. Blood and lung biopsies were taken immediately before OLV, 30 min and 60 min of OLV and after restoration of TLV. Results: Histological analyses revealed that the non-ventilated lung was totally collapsed during OLV. On reventilation, there was clear evidence of vascular congestion and alveolar wall thickening at 30 min after TLV. At 60 min of TLV, there was still vascular congestion. Serum nitrite levels (as an index of nitric oxide production) showed steady decline over the course of the experimental period, reaching a significantly low level on reventilation (compared with baseline levels before OLV). Lung MPO activity (marker of neutrophil sequestration) and serum TNFα levels were not raised during the entire experimental period. Conclusions: These results suggest that there was lung vascular injury after OLV, which was associated with reduced levels of nitric oxide production and not associated with an inflammatory response.

Original languageEnglish (US)
Pages (from-to)16-20
Number of pages5
JournalJournal of Surgical Research
Volume137
Issue number1
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

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One-Lung Ventilation
Lung
Ventilation
Blood Vessels
Nitric Oxide
Tracheotomy
Vascular System Injuries
Lung Injury
Bronchi
Nitrites
Vasoconstriction
Serum
Constriction
Histology
Neutrophils
Swine
Thorax

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Yin, K., Gribbin, E., Emanuel, S., Orndorff, R., Walker, J., Weese, J., & Fallahnejad, M. (2007). Histochemical Alterations in One Lung Ventilation. Journal of Surgical Research, 137(1), 16-20. https://doi.org/10.1016/j.jss.2006.04.038
Yin, Kingsley ; Gribbin, Elizabeth ; Emanuel, Steven ; Orndorff, Rebecca ; Walker, Jean ; Weese, James ; Fallahnejad, Manucher. / Histochemical Alterations in One Lung Ventilation. In: Journal of Surgical Research. 2007 ; Vol. 137, No. 1. pp. 16-20.
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Yin, K, Gribbin, E, Emanuel, S, Orndorff, R, Walker, J, Weese, J & Fallahnejad, M 2007, 'Histochemical Alterations in One Lung Ventilation', Journal of Surgical Research, vol. 137, no. 1, pp. 16-20. https://doi.org/10.1016/j.jss.2006.04.038

Histochemical Alterations in One Lung Ventilation. / Yin, Kingsley; Gribbin, Elizabeth; Emanuel, Steven; Orndorff, Rebecca; Walker, Jean; Weese, James; Fallahnejad, Manucher.

In: Journal of Surgical Research, Vol. 137, No. 1, 01.01.2007, p. 16-20.

Research output: Contribution to journalArticle

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AU - Gribbin, Elizabeth

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AU - Walker, Jean

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AU - Fallahnejad, Manucher

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N2 - Background: One lung ventilation is a commonly performed surgical procedure. Although there have been several reports showing that one-lung ventilation can cause pathophysiological alterations such as pulmonary hypoxic vasoconstriction and intrapulmonary shunting, there have been virtually no reports on the effects of one-lung ventilation on lung histology. Materials and methods: Yorkshire pigs (11-17 kg) were anesthetized, a tracheotomy performed and a tracheal tube inserted. The chest was opened and one lung ventilation (OLV), was induced by clamping of the right main bronchus. OLV was continued for 60 min before the clamp was removed and two lung ventilation (TLV) started. TLV was continued for 30 to 60 min. Blood and lung biopsies were taken immediately before OLV, 30 min and 60 min of OLV and after restoration of TLV. Results: Histological analyses revealed that the non-ventilated lung was totally collapsed during OLV. On reventilation, there was clear evidence of vascular congestion and alveolar wall thickening at 30 min after TLV. At 60 min of TLV, there was still vascular congestion. Serum nitrite levels (as an index of nitric oxide production) showed steady decline over the course of the experimental period, reaching a significantly low level on reventilation (compared with baseline levels before OLV). Lung MPO activity (marker of neutrophil sequestration) and serum TNFα levels were not raised during the entire experimental period. Conclusions: These results suggest that there was lung vascular injury after OLV, which was associated with reduced levels of nitric oxide production and not associated with an inflammatory response.

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Yin K, Gribbin E, Emanuel S, Orndorff R, Walker J, Weese J et al. Histochemical Alterations in One Lung Ventilation. Journal of Surgical Research. 2007 Jan 1;137(1):16-20. https://doi.org/10.1016/j.jss.2006.04.038