Identification and analysis of NADPH-cytochrome p450 reductase in aedes sollicitans (Diptera: Culicidae)

C. Suwanchaichinda, D. Sun, L. B. Brattsten

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

An NADPH-cytochrome P450 reductase (CPR) gene was identified in Aedes sollicitans Walker (Diptera: Culicidae). The open reading frame is 2,040 bp long, encoding a 679-residue polypeptide. Amino acid sequence analysis indicates that the Ae. sollicitans CPR carries conserved ligand-binding domains and belongs to the same phylogenetic group as CPRs in other mosquitoes. The cDNA of the CPR gene was cloned, and the recombinant protein was expressed in Escherichia coli. Cytochrome c reductase activity was detected in the bacterial cytosolic and membrane fractions and in larval microsomes from two New Jersey Ae. sollicitans populations, indicating that the Ae. sollicitans CPR is enzymatically functional and closely related to that in other dipterans.

Original languageEnglish (US)
Pages (from-to)958-963
Number of pages6
JournalJournal of medical entomology
Volume51
Issue number5
DOIs
StatePublished - Sep 2014

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Keywords

  • Aedes sollicitans
  • activity
  • cytochrome P450 reductase
  • salt marsh mosquito
  • sequence

Fingerprint

Dive into the research topics of 'Identification and analysis of NADPH-cytochrome p450 reductase in aedes sollicitans (Diptera: Culicidae)'. Together they form a unique fingerprint.

Cite this