Identification and Structural Modeling of the RNA Polymerase Omega Subunits in Chlamydiae and Other Obligate Intracellular Bacteria

TY - JOUR

T1 - Identification and Structural Modeling of the RNA Polymerase Omega Subunits in Chlamydiae and Other Obligate Intracellular Bacteria

AU - Cheng, Andrew

AU - Wan, Danny

AU - Ghatak, Arkaprabha

AU - Wang, Chengyuan

AU - Feng, Deyu

AU - Fondell, Joseph D.

AU - Ebright, Richard H.

AU - Fan, Huizhou

N1 - Publisher Copyright: © 2023 Cheng et al.

PY - 2023/1

Y1 - 2023/1

N2 - Gene transcription in bacteria is carried out by the multisubunit RNA polymerase (RNAP),which is composed of a catalytic core enzyme anda promoter-recognizings factor. The core enzyme comprises two a subunits, one b subunit, one b9 subunit, and one v subunit. Thev subunit plays critical roles in the assembly of the core enzyme and other cellular functions, including the regulation of bacterial growth, the stress response, and biofilm formation. However, the identity of an v subunit for the obligate intracellular bacterium Chlamydia has not previously been determined. Here, we report the identification of the hypothetical protein CTL0286 as the probable chlamydial v subunit based on sequence, synteny, and AlphaFold and AlphaFold-Multimer three-dimensional-structure predictions. Our findings indicate that CTL0286 functions as the missing v subunit of chlamydial RNAP. Our extended analysis also indicates that all obligate intracellular bacteria havev orthologs.

AB - Gene transcription in bacteria is carried out by the multisubunit RNA polymerase (RNAP),which is composed of a catalytic core enzyme anda promoter-recognizings factor. The core enzyme comprises two a subunits, one b subunit, one b9 subunit, and one v subunit. Thev subunit plays critical roles in the assembly of the core enzyme and other cellular functions, including the regulation of bacterial growth, the stress response, and biofilm formation. However, the identity of an v subunit for the obligate intracellular bacterium Chlamydia has not previously been determined. Here, we report the identification of the hypothetical protein CTL0286 as the probable chlamydial v subunit based on sequence, synteny, and AlphaFold and AlphaFold-Multimer three-dimensional-structure predictions. Our findings indicate that CTL0286 functions as the missing v subunit of chlamydial RNAP. Our extended analysis also indicates that all obligate intracellular bacteria havev orthologs.

KW - Chlamydia

KW - RNA polymerases

KW - omega subunit

UR - http://www.scopus.com/inward/record.url?scp=85149153951&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85149153951&partnerID=8YFLogxK

U2 - 10.1128/mbio.03499-22

DO - 10.1128/mbio.03499-22

M3 - Article

C2 - 36719197

SN - 2161-2129

VL - 14

JO - mBio

JF - mBio

IS - 1

ER -