Imaging infections with antibodies. A quantitative autoradiographic analysis

Eric M. Morrel, Ronald G. Tompkins, Alan J. Fischman, Robert A. Wilkinson, Martin Yarmush

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Radiolabeled IgG has recently been demonstrated to effectively image infections. A potential but unproven mechanism for this localization is the specific binding of IgG to Fc receptors on the surface of inflammatory cells in infections. In an animal model of soft tissue infection, quantitative autoradiography was used to measure 125I-labeled IgG and albumin in tissues with a spatial resolution sufficient to associate these proteins with cellular morphology. Gamma camera images at 24 h localized the infection with target-to-background ratios of 2.2 ± 0.5 for IgG and 2.3 ± 1.0 for albumin (mean ± SD). Using quantitative autoradiography at 1 h post-injection, significantly higher concentrations were found in infected thighs of 2-4% of initial plasma concentrations (CPo) as compared to 0.2-0.3% of CPo in noninfected thighs (P < 0.05); at 24 h post-injection, higher concentrations (7-8% of CPo) were found in infected thighs. Radiolabeled proteins were not inflammatory cell associated and were localized primarily within the edematous interstital spaces of the infection.

Original languageEnglish (US)
Pages (from-to)39-48
Number of pages10
JournalJournal of Immunological Methods
Volume130
Issue number1
DOIs
StatePublished - Jun 12 1990

Fingerprint

Immunoglobulin G
Thigh
Antibodies
Infection
Autoradiography
Albumins
Soft Tissue Infections
Injections
Gamma Cameras
Fc Receptors
Proteins
Animal Models

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Keywords

  • Albumin
  • Escherichia coli infection
  • Iodine radioisotope
  • Polyclonal antibody
  • Quantitative autoradiography

Cite this

Morrel, Eric M. ; Tompkins, Ronald G. ; Fischman, Alan J. ; Wilkinson, Robert A. ; Yarmush, Martin. / Imaging infections with antibodies. A quantitative autoradiographic analysis. In: Journal of Immunological Methods. 1990 ; Vol. 130, No. 1. pp. 39-48.
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abstract = "Radiolabeled IgG has recently been demonstrated to effectively image infections. A potential but unproven mechanism for this localization is the specific binding of IgG to Fc receptors on the surface of inflammatory cells in infections. In an animal model of soft tissue infection, quantitative autoradiography was used to measure 125I-labeled IgG and albumin in tissues with a spatial resolution sufficient to associate these proteins with cellular morphology. Gamma camera images at 24 h localized the infection with target-to-background ratios of 2.2 ± 0.5 for IgG and 2.3 ± 1.0 for albumin (mean ± SD). Using quantitative autoradiography at 1 h post-injection, significantly higher concentrations were found in infected thighs of 2-4{\%} of initial plasma concentrations (CPo) as compared to 0.2-0.3{\%} of CPo in noninfected thighs (P < 0.05); at 24 h post-injection, higher concentrations (7-8{\%} of CPo) were found in infected thighs. Radiolabeled proteins were not inflammatory cell associated and were localized primarily within the edematous interstital spaces of the infection.",
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Imaging infections with antibodies. A quantitative autoradiographic analysis. / Morrel, Eric M.; Tompkins, Ronald G.; Fischman, Alan J.; Wilkinson, Robert A.; Yarmush, Martin.

In: Journal of Immunological Methods, Vol. 130, No. 1, 12.06.1990, p. 39-48.

Research output: Contribution to journalArticle

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