Implementation of the CRISPR-Cas9 system in fission yeast

Jake Z. Jacobs, Keith M. Ciccaglione, Vincent Tournier, Mikel Zaratiegui

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of K RNA (rrk1) and a ribozyme to produce the targeting guide RNA. Together with constitutive expression of Cas9, this system achieves selection-free specific mutagenesis with efficiencies approaching 100%. The rrk1 CRISPR-Cas9 method enables rapid and efficient genome manipulation and unlocks the CRISPR toolset for use in fission yeast.

Original languageEnglish (US)
Article number5344
JournalNature communications
Volume5
DOIs
StatePublished - 2014

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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