In vivo regulation of human skeletal muscle gene expression by thyroid hormone

Karine Clément, Nathalie Viguerie, Maximilian Diehn, Ash Alizadeh, Pierre Barbe, Claire Thalamas, John D. Storey, Patrick O. Brown, Greg S. Barsh, Dominique Langin

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Thyroid hormones are key regulators of metabolism that modulate transcription via nuclear receptors. Hyperthyroidism is associated with increased metabolic rate, protein breakdown, and weight loss. Although the molecular actions of thyroid hormones have been studied thoroughly, their pleiotropic effects are mediated by complex changes in expression of an unknown number of target genes. Here, we measured patterns of skeletal muscle gene expression in five healthy men treated for 14 days with 75 μg of triiodothyronine, using 24,000 cDNA element microarrays. To analyze the data, we used a new statistical method that identifies significant changes in expression and estimates the false discovery rate. The 381 up-regulated genes were involved in a wide range of cellular functions including transcriptional control, mRNA maturation, protein turnover, signal transduction, cellular trafficking, and energy metabolism. Only two genes were down-regulated. Most of the genes are novel targets of thyroid hormone. Cluster analysis of triiodothyronine-regulated gene expression among 19 different human tissues or cell lines revealed sets of coregulated genes that serve similar biologic functions. These results define molecular signatures that help to understand the physiology and pathophysiology of thyroid hormone action.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalGenome Research
Volume12
Issue number2
DOIs
StatePublished - Mar 4 2002

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Thyroid Hormones
Skeletal Muscle
Gene Expression
Genes
Triiodothyronine
Hyperthyroidism
Cytoplasmic and Nuclear Receptors
Oligonucleotide Array Sequence Analysis
Energy Metabolism
Cluster Analysis
Weight Loss
Signal Transduction
Proteins
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)
  • Genetics

Cite this

Clément, K., Viguerie, N., Diehn, M., Alizadeh, A., Barbe, P., Thalamas, C., ... Langin, D. (2002). In vivo regulation of human skeletal muscle gene expression by thyroid hormone. Genome Research, 12(2), 281-291. https://doi.org/10.1101/gr.207702
Clément, Karine ; Viguerie, Nathalie ; Diehn, Maximilian ; Alizadeh, Ash ; Barbe, Pierre ; Thalamas, Claire ; Storey, John D. ; Brown, Patrick O. ; Barsh, Greg S. ; Langin, Dominique. / In vivo regulation of human skeletal muscle gene expression by thyroid hormone. In: Genome Research. 2002 ; Vol. 12, No. 2. pp. 281-291.
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Clément, K, Viguerie, N, Diehn, M, Alizadeh, A, Barbe, P, Thalamas, C, Storey, JD, Brown, PO, Barsh, GS & Langin, D 2002, 'In vivo regulation of human skeletal muscle gene expression by thyroid hormone', Genome Research, vol. 12, no. 2, pp. 281-291. https://doi.org/10.1101/gr.207702

In vivo regulation of human skeletal muscle gene expression by thyroid hormone. / Clément, Karine; Viguerie, Nathalie; Diehn, Maximilian; Alizadeh, Ash; Barbe, Pierre; Thalamas, Claire; Storey, John D.; Brown, Patrick O.; Barsh, Greg S.; Langin, Dominique.

In: Genome Research, Vol. 12, No. 2, 04.03.2002, p. 281-291.

Research output: Contribution to journalArticle

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Clément K, Viguerie N, Diehn M, Alizadeh A, Barbe P, Thalamas C et al. In vivo regulation of human skeletal muscle gene expression by thyroid hormone. Genome Research. 2002 Mar 4;12(2):281-291. https://doi.org/10.1101/gr.207702