Increasing and decreasing protein stability

Effects of revertant substitutions on the thermal denaturation of phage λ repressor

Michael H. Hecht, Kathleen M. Hehir, Hillary C.M. Nelson, Julian M. Sturtevant, Robert T. Sauer

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The thermal denaturations of five revertant λ repressors containing single amino acid substitutions in their N‐terminal domains have been studied by differential scanning calorimetry. Two substitutions slightly decrease stability, and the remaining three render the protein more stable than wild type. The Gly48 → Asn and Gly48 → Ser proteins are 4°C more stable than wild type. These two substitutions replace an α helical residue, and in each case a poor helix forming residue, glycine, is replaced by a residue with a higher helical propensity. We also present data showing that one revertant, Tyr22 → Phe, has reduced operator DNA binding affinity despite its enhanced stability.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalJournal of Cellular Biochemistry
Volume29
Issue number3
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

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Denaturation
Bacteriophages
Protein Stability
Substitution reactions
Hot Temperature
Differential Scanning Calorimetry
Amino Acid Substitution
Glycine
Proteins
Differential scanning calorimetry
DNA
Amino Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Hecht, Michael H. ; Hehir, Kathleen M. ; Nelson, Hillary C.M. ; Sturtevant, Julian M. ; Sauer, Robert T. / Increasing and decreasing protein stability : Effects of revertant substitutions on the thermal denaturation of phage λ repressor. In: Journal of Cellular Biochemistry. 1985 ; Vol. 29, No. 3. pp. 217-224.
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Increasing and decreasing protein stability : Effects of revertant substitutions on the thermal denaturation of phage λ repressor. / Hecht, Michael H.; Hehir, Kathleen M.; Nelson, Hillary C.M.; Sturtevant, Julian M.; Sauer, Robert T.

In: Journal of Cellular Biochemistry, Vol. 29, No. 3, 01.01.1985, p. 217-224.

Research output: Contribution to journalArticle

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