Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model

R. E. Monyak, D. Emerson, B. P. Schoenfeld, X. Zheng, D. B. Chambers, C. Rosenfelt, S. Langer, P. Hinchey, C. H. Choi, T. V. McDonald, F. V. Bolduc, A. Sehgal, S. M.J. McBride, T. A. Jongens

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Fragile X syndrome (FXS) is an undertreated neurodevelopmental disorder characterized by low intelligence quotent and a wide range of other symptoms including disordered sleep and autism. Although FXS is the most prevalent inherited cause of intellectual disability, its mechanistic underpinnings are not well understood. Using Drosophila as a model of FXS, we showed that select expression of dfmr1 in the insulin-producing cells (IPCs) of the brain was sufficient to restore normal circadian behavior and to rescue the memory deficits in the fragile X mutant fly. Examination of the insulin signaling (IS) pathway revealed elevated levels of Drosophila insulin-like peptide 2 (Dilp2) in the IPCs and elevated IS in the dfmr1 mutant brain. Consistent with a causal role for elevated IS in dfmr1 mutant phenotypes, the expression of dfmr1 specifically in the IPCs reduced IS, and genetic reduction of the insulin pathway also led to amelioration of circadian and memory defects. Furthermore, we showed that treatment with the FDA-approved drug metformin also rescued memory. Finally, we showed that reduction of IS is required at different time points to rescue circadian behavior and memory. Our results indicate that insulin misregulation underlies the circadian and cognitive phenotypes displayed by the Drosophila fragile X model, and thus reveal a metabolic pathway that can be targeted by new and already approved drugs to treat fragile X patients.

Original languageEnglish (US)
Pages (from-to)1140-1148
Number of pages9
JournalMolecular psychiatry
Volume22
Issue number8
DOIs
StatePublished - Aug 1 2017

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Drosophila
Insulin
Fragile X Syndrome
Phenotype
Metformin
Memory Disorders
Brain
Autistic Disorder
Metabolic Networks and Pathways
Intelligence
Diptera
Intellectual Disability
Pharmaceutical Preparations
Sleep
Peptides

All Science Journal Classification (ASJC) codes

  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience
  • Molecular Biology

Cite this

Monyak, R. E., Emerson, D., Schoenfeld, B. P., Zheng, X., Chambers, D. B., Rosenfelt, C., ... Jongens, T. A. (2017). Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model. Molecular psychiatry, 22(8), 1140-1148. https://doi.org/10.1038/mp.2016.51
Monyak, R. E. ; Emerson, D. ; Schoenfeld, B. P. ; Zheng, X. ; Chambers, D. B. ; Rosenfelt, C. ; Langer, S. ; Hinchey, P. ; Choi, C. H. ; McDonald, T. V. ; Bolduc, F. V. ; Sehgal, A. ; McBride, S. M.J. ; Jongens, T. A. / Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model. In: Molecular psychiatry. 2017 ; Vol. 22, No. 8. pp. 1140-1148.
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Monyak, RE, Emerson, D, Schoenfeld, BP, Zheng, X, Chambers, DB, Rosenfelt, C, Langer, S, Hinchey, P, Choi, CH, McDonald, TV, Bolduc, FV, Sehgal, A, McBride, SMJ & Jongens, TA 2017, 'Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model', Molecular psychiatry, vol. 22, no. 8, pp. 1140-1148. https://doi.org/10.1038/mp.2016.51

Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model. / Monyak, R. E.; Emerson, D.; Schoenfeld, B. P.; Zheng, X.; Chambers, D. B.; Rosenfelt, C.; Langer, S.; Hinchey, P.; Choi, C. H.; McDonald, T. V.; Bolduc, F. V.; Sehgal, A.; McBride, S. M.J.; Jongens, T. A.

In: Molecular psychiatry, Vol. 22, No. 8, 01.08.2017, p. 1140-1148.

Research output: Contribution to journalArticle

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AU - Monyak, R. E.

AU - Emerson, D.

AU - Schoenfeld, B. P.

AU - Zheng, X.

AU - Chambers, D. B.

AU - Rosenfelt, C.

AU - Langer, S.

AU - Hinchey, P.

AU - Choi, C. H.

AU - McDonald, T. V.

AU - Bolduc, F. V.

AU - Sehgal, A.

AU - McBride, S. M.J.

AU - Jongens, T. A.

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Monyak RE, Emerson D, Schoenfeld BP, Zheng X, Chambers DB, Rosenfelt C et al. Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model. Molecular psychiatry. 2017 Aug 1;22(8):1140-1148. https://doi.org/10.1038/mp.2016.51