Abstract
We studied the interaction between tRNA and three polyamine analogues (1,11-diamino-4,8-diazaundecane .HCl (333), 3,7,11,15-tetrazaheptadecane.HCl (BE-333), and 3,7,11,15,19-pentazahenicosane.HCl (BE-3333)) using FTIR, UV-visible, and CD spectroscopic methods. Spectroscopic evidence showed that polyamine analogues bound tRNA via guanine N7, adenine, uracil O2, and the backbone phosphate (PO2 -) groups, while the most reactive sites for biogenic polyamines were guanine N7/O6, adenine N7, uracil O2, and sugar 2′-OH groups as well as the backbone phosphate group. The binding constants of polyamine analogue - tRNA recognition were lower than those of the biogenic polyamine - tRNA complexes, with K333 = 2.8 (±0.5) × 104, KBE-333 = 3.7 (±0.7) × 104, KBE-3333 = 4.0 (±0.9) × 104, Kspm = 8.7 (±0.9) × 105, Kspd = 6.1 (±0.7 × 105, and Kput = 1.0 (±0.3) × 105 mol/L. tRNA remained in the A-family conformation; however, it aggregated at high polyamine analogue concentrations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 621-630 |
| Number of pages | 10 |
| Journal | Biochemistry and Cell Biology |
| Volume | 87 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 2009 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Biochemistry
- Cell Biology
Keywords
- Binding constant
- DNA aggregation
- DNA stability
- FTIR spectroscopy
- Polyamines
- Preferential binding site
- tRNA
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Interaction of tRNA with antitumor polyamine analogues. / N'soukpoé-Kossi, C. N.; Ahmed Ouameur, A.; Thomas, T.; Thomas, Thekkumkat; Tajmir-Riahi, H. A.
In: Biochemistry and Cell Biology, Vol. 87, No. 4, 01.08.2009, p. 621-630.Research output: Contribution to journal › Article
TY - JOUR
T1 - Interaction of tRNA with antitumor polyamine analogues
AU - N'soukpoé-Kossi, C. N.
AU - Ahmed Ouameur, A.
AU - Thomas, T.
AU - Thomas, Thekkumkat
AU - Tajmir-Riahi, H. A.
PY - 2009/8/1
Y1 - 2009/8/1
N2 - We studied the interaction between tRNA and three polyamine analogues (1,11-diamino-4,8-diazaundecane .HCl (333), 3,7,11,15-tetrazaheptadecane.HCl (BE-333), and 3,7,11,15,19-pentazahenicosane.HCl (BE-3333)) using FTIR, UV-visible, and CD spectroscopic methods. Spectroscopic evidence showed that polyamine analogues bound tRNA via guanine N7, adenine, uracil O2, and the backbone phosphate (PO2 -) groups, while the most reactive sites for biogenic polyamines were guanine N7/O6, adenine N7, uracil O2, and sugar 2′-OH groups as well as the backbone phosphate group. The binding constants of polyamine analogue - tRNA recognition were lower than those of the biogenic polyamine - tRNA complexes, with K333 = 2.8 (±0.5) × 104, KBE-333 = 3.7 (±0.7) × 104, KBE-3333 = 4.0 (±0.9) × 104, Kspm = 8.7 (±0.9) × 105, Kspd = 6.1 (±0.7 × 105, and Kput = 1.0 (±0.3) × 105 mol/L. tRNA remained in the A-family conformation; however, it aggregated at high polyamine analogue concentrations.
AB - We studied the interaction between tRNA and three polyamine analogues (1,11-diamino-4,8-diazaundecane .HCl (333), 3,7,11,15-tetrazaheptadecane.HCl (BE-333), and 3,7,11,15,19-pentazahenicosane.HCl (BE-3333)) using FTIR, UV-visible, and CD spectroscopic methods. Spectroscopic evidence showed that polyamine analogues bound tRNA via guanine N7, adenine, uracil O2, and the backbone phosphate (PO2 -) groups, while the most reactive sites for biogenic polyamines were guanine N7/O6, adenine N7, uracil O2, and sugar 2′-OH groups as well as the backbone phosphate group. The binding constants of polyamine analogue - tRNA recognition were lower than those of the biogenic polyamine - tRNA complexes, with K333 = 2.8 (±0.5) × 104, KBE-333 = 3.7 (±0.7) × 104, KBE-3333 = 4.0 (±0.9) × 104, Kspm = 8.7 (±0.9) × 105, Kspd = 6.1 (±0.7 × 105, and Kput = 1.0 (±0.3) × 105 mol/L. tRNA remained in the A-family conformation; however, it aggregated at high polyamine analogue concentrations.
KW - Binding constant
KW - DNA aggregation
KW - DNA stability
KW - FTIR spectroscopy
KW - Polyamines
KW - Preferential binding site
KW - tRNA
UR - http://www.scopus.com/inward/record.url?scp=68049097880&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68049097880&partnerID=8YFLogxK
U2 - https://doi.org/10.1139/O09-036
DO - https://doi.org/10.1139/O09-036
M3 - Article
C2 - 19767825
VL - 87
SP - 621
EP - 630
JO - Biochemistry and Cell Biology
JF - Biochemistry and Cell Biology
SN - 0829-8211
IS - 4
ER -