@inbook{f8e5b012adac48cabd8d10db5099ffef,
title = "Interferometric Scattering Microscopy for the Study of Molecular Motors",
abstract = "Our understanding of molecular motor function has been greatly improved by the development of imaging modalities, which enable real-time observation of their motion at the single-molecule level. Here, we describe the use of a new method, interferometric scattering microscopy, for the investigation of motor protein dynamics by attaching and tracking the motion of metallic nanoparticle labels as small as 20 nm diameter. Using myosin-5, kinesin-1, and dynein as examples, we describe the basic assays, labeling strategies, and principles of data analysis. Our approach is relevant not only for motor protein dynamics but also provides a general tool for single-particle tracking with high spatiotemporal precision, which overcomes the limitations of single-molecule fluorescence methods.",
keywords = "Dynein, High speed, Interferometric scattering microscopy, Kinesin, Molecular motors, Myosin, Single molecule, Single-particle tracking",
author = "J. Andrecka and Y. Takagi and Mickolajczyk, \{K. J.\} and Lippert, \{L. G.\} and Sellers, \{J. R.\} and Hancock, \{W. O.\} and Goldman, \{Y. E.\} and P. Kukura",
note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
doi = "10.1016/bs.mie.2016.08.016",
language = "American English",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "517--539",
booktitle = "Methods in Enzymology",
address = "United States",
}