Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells

Bénédicte Danis, Thaddeus C. George, Stanislas Goriely, Binita Dutta, Joëlle Renneson, Laurent Gatto, Patricia Fitzgerald-Bocarsly, Arnaud Marchant, Michel Goldman, Fabienne Willems, Dominique de Wit

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Plasmacytoid dendritic cells (pDC) are specialized in massive production of type I interferons (IFN) upon viral infections. Activation of IFN regulatory factor (IRF)-7 is critically required for the synthesis of type I IFN in pDC. IRF-7 is highly expressed by resting pDC and translocates into the nucleus to initiate type I IFN transcription. In a previous work, we observed an impaired IFN-α production in enriched cord blood pDC following a TLR9 stimulation using CpG oligonucleotides. Herein, we show that highly purified pDC from cord blood exhibit a profound defect in their capacity to produce IFN-α/β in response to TLR9 as well as to TLR7 ligation or human CMV or HSV-1 exposure. Microarray experiments indicate that expression of the majority of type I IFN subtypes induced by a TLR7 agonist is reduced in cord blood pDC. We next demonstrated a reduced nuclear translocation of IRF-7 in cord blood pDC following CpG and HSV stimulation as compared to adult pDC. We conclude that impaired IRF-7 translocation in cord blood pDC is associated with defective expression of type I IFN genes. Our data provide a molecular understanding for the decreased ability of cord blood pDC to produce type I IFN upon viral stimulation.

Original languageEnglish (US)
Pages (from-to)507-517
Number of pages11
JournalEuropean Journal of Immunology
Volume38
Issue number2
DOIs
StatePublished - Feb 1 2008

Fingerprint

Interferon Regulatory Factor-7
Fetal Blood
Dendritic Cells
Interferon Type I
Interferons
Human Herpesvirus 1
Virus Diseases
Oligonucleotides
Ligation

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Keywords

  • Cord blood
  • Dendritic cell
  • IFN-α
  • Interferon regulatory factor 7
  • Newborn

Cite this

Danis, B., George, T. C., Goriely, S., Dutta, B., Renneson, J., Gatto, L., ... de Wit, D. (2008). Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells. European Journal of Immunology, 38(2), 507-517. https://doi.org/10.1002/eji.200737760
Danis, Bénédicte ; George, Thaddeus C. ; Goriely, Stanislas ; Dutta, Binita ; Renneson, Joëlle ; Gatto, Laurent ; Fitzgerald-Bocarsly, Patricia ; Marchant, Arnaud ; Goldman, Michel ; Willems, Fabienne ; de Wit, Dominique. / Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells. In: European Journal of Immunology. 2008 ; Vol. 38, No. 2. pp. 507-517.
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Danis, B, George, TC, Goriely, S, Dutta, B, Renneson, J, Gatto, L, Fitzgerald-Bocarsly, P, Marchant, A, Goldman, M, Willems, F & de Wit, D 2008, 'Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells', European Journal of Immunology, vol. 38, no. 2, pp. 507-517. https://doi.org/10.1002/eji.200737760

Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells. / Danis, Bénédicte; George, Thaddeus C.; Goriely, Stanislas; Dutta, Binita; Renneson, Joëlle; Gatto, Laurent; Fitzgerald-Bocarsly, Patricia; Marchant, Arnaud; Goldman, Michel; Willems, Fabienne; de Wit, Dominique.

In: European Journal of Immunology, Vol. 38, No. 2, 01.02.2008, p. 507-517.

Research output: Contribution to journalArticle

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AU - Danis, Bénédicte

AU - George, Thaddeus C.

AU - Goriely, Stanislas

AU - Dutta, Binita

AU - Renneson, Joëlle

AU - Gatto, Laurent

AU - Fitzgerald-Bocarsly, Patricia

AU - Marchant, Arnaud

AU - Goldman, Michel

AU - Willems, Fabienne

AU - de Wit, Dominique

PY - 2008/2/1

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