Iron-restricted Mycobacterium tuberculosis exports pathogenicity factors packed in extracellular vesicles

Nishant Sharma; Nevadita Sharma; Ashis Biswas; Shamba Gupta; Assirbad Behura; Gloria Marcela Rodriguez

doi:10.1371/journal.pone.0324919

Iron-restricted Mycobacterium tuberculosis exports pathogenicity factors packed in extracellular vesicles

Nishant Sharma
, Nevadita Sharma
, Ashis Biswas
, Shamba Gupta
, Assirbad Behura
, Gloria Marcela Rodriguez

New Jersey Medical School (NJMS), Public Health Research Institute Center

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

Abstract

Mycobacterium tuberculosis, the pathogen responsible for human tuberculosis, responds to iron limitation by increasing the production of extracellular vesicles. This study examined the protein composition of induced M. tuberculosis extracellular membrane vesicles using chromatography coupled with mass spectrometry. The results revealed that vesicles contain key pathogenicity factors, including proteins that enhance bacterial survival, immune evasion, and inflammation. These findings deepen our understanding of the potential role of extracellular vesicles in M. tuberculosis-host interactions. The data can also aid in identifying new biomarkers of infection and developing vesicle-based, culture-independent TB diagnostic platforms.

Original language	American English
Article number	e0324919
Journal	PLoS One
Volume	20
Issue number	5 May
DOIs	https://doi.org/10.1371/journal.pone.0324919
State	Published - May 2025

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abstract = "Mycobacterium tuberculosis, the pathogen responsible for human tuberculosis, responds to iron limitation by increasing the production of extracellular vesicles. This study examined the protein composition of induced M. tuberculosis extracellular membrane vesicles using chromatography coupled with mass spectrometry. The results revealed that vesicles contain key pathogenicity factors, including proteins that enhance bacterial survival, immune evasion, and inflammation. These findings deepen our understanding of the potential role of extracellular vesicles in M. tuberculosis-host interactions. The data can also aid in identifying new biomarkers of infection and developing vesicle-based, culture-independent TB diagnostic platforms.",

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AU - Behura, Assirbad

AU - Rodriguez, Gloria Marcela

N1 - Publisher Copyright: © 2025 Sharma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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AB - Mycobacterium tuberculosis, the pathogen responsible for human tuberculosis, responds to iron limitation by increasing the production of extracellular vesicles. This study examined the protein composition of induced M. tuberculosis extracellular membrane vesicles using chromatography coupled with mass spectrometry. The results revealed that vesicles contain key pathogenicity factors, including proteins that enhance bacterial survival, immune evasion, and inflammation. These findings deepen our understanding of the potential role of extracellular vesicles in M. tuberculosis-host interactions. The data can also aid in identifying new biomarkers of infection and developing vesicle-based, culture-independent TB diagnostic platforms.

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Iron-restricted Mycobacterium tuberculosis exports pathogenicity factors packed in extracellular vesicles

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