Isolation of a mutant that changes genomic packaging specificity in φ6

Shiroh Onodera, Xueying Qiao, Jian Qiao, Leonard Mindich

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Bacteriophage φ6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. Plus strand transcripts of the segments are packaged in a serially dependent fashion in which S can package alone, M depends on S, and L depends on S and M. We have isolated a mutant form of the virus in the carrier state that has lost segment S. This finding presented an apparent anomaly with respect to the packaging program. Sequencing of gene 1 of segment L in this virus showed a translational change of arginine to glycine at the 14th position. Procapsids prepared from cDNA containing this mutation show behavior in in vitro packaging that is consistent with the phenotype of the mutant virus. The procapsids are able to package segment S alone, but this RNA is present in reduced amounts when the other segments are present. Segments M and L package without dependence on segment S. The mutant virus appears to produce procapsids that are at the second stage of the packaging program.

Original languageEnglish (US)
Pages (from-to)438-442
Number of pages5
JournalVirology
Volume252
Issue number2
DOIs
StatePublished - Dec 20 1998

Fingerprint

Product Packaging
Viruses
Carrier State
Double-Stranded RNA
Bacteriophages
Glycine
Arginine
Complementary DNA
Genome
RNA
Phenotype
Mutation
Genes

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Onodera, Shiroh ; Qiao, Xueying ; Qiao, Jian ; Mindich, Leonard. / Isolation of a mutant that changes genomic packaging specificity in φ6. In: Virology. 1998 ; Vol. 252, No. 2. pp. 438-442.
@article{4bb42a17841744f39bda9c88793d3143,
title = "Isolation of a mutant that changes genomic packaging specificity in φ6",
abstract = "Bacteriophage φ6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. Plus strand transcripts of the segments are packaged in a serially dependent fashion in which S can package alone, M depends on S, and L depends on S and M. We have isolated a mutant form of the virus in the carrier state that has lost segment S. This finding presented an apparent anomaly with respect to the packaging program. Sequencing of gene 1 of segment L in this virus showed a translational change of arginine to glycine at the 14th position. Procapsids prepared from cDNA containing this mutation show behavior in in vitro packaging that is consistent with the phenotype of the mutant virus. The procapsids are able to package segment S alone, but this RNA is present in reduced amounts when the other segments are present. Segments M and L package without dependence on segment S. The mutant virus appears to produce procapsids that are at the second stage of the packaging program.",
author = "Shiroh Onodera and Xueying Qiao and Jian Qiao and Leonard Mindich",
year = "1998",
month = "12",
day = "20",
doi = "https://doi.org/10.1006/viro.1998.9479",
language = "English (US)",
volume = "252",
pages = "438--442",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

Isolation of a mutant that changes genomic packaging specificity in φ6. / Onodera, Shiroh; Qiao, Xueying; Qiao, Jian; Mindich, Leonard.

In: Virology, Vol. 252, No. 2, 20.12.1998, p. 438-442.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation of a mutant that changes genomic packaging specificity in φ6

AU - Onodera, Shiroh

AU - Qiao, Xueying

AU - Qiao, Jian

AU - Mindich, Leonard

PY - 1998/12/20

Y1 - 1998/12/20

N2 - Bacteriophage φ6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. Plus strand transcripts of the segments are packaged in a serially dependent fashion in which S can package alone, M depends on S, and L depends on S and M. We have isolated a mutant form of the virus in the carrier state that has lost segment S. This finding presented an apparent anomaly with respect to the packaging program. Sequencing of gene 1 of segment L in this virus showed a translational change of arginine to glycine at the 14th position. Procapsids prepared from cDNA containing this mutation show behavior in in vitro packaging that is consistent with the phenotype of the mutant virus. The procapsids are able to package segment S alone, but this RNA is present in reduced amounts when the other segments are present. Segments M and L package without dependence on segment S. The mutant virus appears to produce procapsids that are at the second stage of the packaging program.

AB - Bacteriophage φ6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. Plus strand transcripts of the segments are packaged in a serially dependent fashion in which S can package alone, M depends on S, and L depends on S and M. We have isolated a mutant form of the virus in the carrier state that has lost segment S. This finding presented an apparent anomaly with respect to the packaging program. Sequencing of gene 1 of segment L in this virus showed a translational change of arginine to glycine at the 14th position. Procapsids prepared from cDNA containing this mutation show behavior in in vitro packaging that is consistent with the phenotype of the mutant virus. The procapsids are able to package segment S alone, but this RNA is present in reduced amounts when the other segments are present. Segments M and L package without dependence on segment S. The mutant virus appears to produce procapsids that are at the second stage of the packaging program.

UR - http://www.scopus.com/inward/record.url?scp=0032567311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032567311&partnerID=8YFLogxK

U2 - https://doi.org/10.1006/viro.1998.9479

DO - https://doi.org/10.1006/viro.1998.9479

M3 - Article

VL - 252

SP - 438

EP - 442

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -