Isolation of lipid droplets from cells by density gradient centrifugation

Dawn L. Brasaemle, Nathan E. Wolins

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.

Original languageEnglish (US)
Pages (from-to)3.15.1-3.15.13.
JournalCurrent Protocols in Cell Biology
Volume2016
DOIs
StatePublished - 2016

All Science Journal Classification (ASJC) codes

  • Cell Biology

Keywords

  • Cell cultures
  • Density-gradient centrifugation
  • Isolation of lipid droplets
  • Mammalian
  • Preparation of lipid droplets by centrifugation

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