IV. Molecular biology of S-layers

Hubert Bahl, Holger Scholz, Nicolas Bayan, Mohamed Chami, Gérard Leblon, Thaddée Gulik-Krzywicki, Emanuel Shechter, Agnés Fouet, Stéphane Mesnage, Evelyne Tosi-Couture, Pierre Gounon, Michèle Mock, Everly Conway De Macario, Alberto J.L. Macario, Luis A. Fernández-Herrero, Garbiñe Olabarría, José Berenguer, Martin Blaser, Beatrix Kuen, Werner Lubitz & 10 others Margit Sára, Peter H. Pouwels, Carin P.A.M. Kolen, Hein J. Boot, Airi Palva, Michaela Truppe, Stephan Howorka, Gerhard Schroll, Sonja Lechleitner, Stephanie Resch

Research output: Contribution to journalReview article

52 Citations (Scopus)

Abstract

In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is considerable variety in the S-layer composition, which was elucidated by sequence analysis of the corresponding genes. In Corynebacterium glutamicum one major cell wall protein is responsible for the formation of a highly ordered, hexagonal array. In contrast, two abundant surface proteins form the S-layer of Bacillus anthracis. Each protein possesses three S-layer homology motifs and one protein could be a virulence factor. The antigenic diversity and ABC transporters are important features, which have been studied in methanogenic archaea. The expression of the S-layer components is controlled by three genes in the case of Thermus thermophilus. One has repressor activity on the S-layer gene promoter, the second codes for the S-layer protein. The rearrangement by reciprocal recombination was investigated in Campylobacter fetus. 7-8 S-layer proteins with a high degree of homology at the 5' and 3' ends were found. Environmental changes influence the surface properties of Bacillus stearothermophilus. Depending on oxygen supply, this species produces different S-layer proteins. Finaly, the molecular bases for some applications are discussed. Recombinant S-layer fusion proteins have been designed for biotechnology.

Original languageEnglish (US)
Pages (from-to)47-98
Number of pages52
JournalFEMS Microbiology Reviews
Volume20
Issue number1-2
DOIs
StatePublished - Jun 1 1997

Fingerprint

Molecular Biology
Antigenic Variation
Campylobacter fetus
Corynebacterium glutamicum
Genes
Thermus thermophilus
Geobacillus stearothermophilus
Bacillus anthracis
Amino Acid Motifs
ATP-Binding Cassette Transporters
Surface Properties
Gene Rearrangement
Protein S
Archaea
Virulence Factors
Biotechnology
Cell Wall
Genetic Recombination
Sequence Analysis
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Microbiology

Keywords

  • ABC transporter
  • Antigenic diversity
  • Bacillus anthracis
  • Bacillus stearothermophilus
  • Corynebacterium glutamicum
  • EAI protein
  • Gene regulation
  • Heterologous expression
  • Lactobacilli
  • Lactobacillus brevis
  • Methanogenic archaea
  • Methanosarcina mazei
  • Oxygen stress
  • PS2 protein
  • S-layer
  • S-layer gene
  • S-layer protein
  • S-layer structure-function relationship
  • S-layer variation
  • SLH motif
  • Sap protein
  • Sbs gene
  • SbsA/SbsB fusion protein
  • Secretion of β-lactamase
  • Slp gene
  • SlpA gene
  • Thermus thermophilus
  • cspB gene

Cite this

Bahl, H., Scholz, H., Bayan, N., Chami, M., Leblon, G., Gulik-Krzywicki, T., ... Resch, S. (1997). IV. Molecular biology of S-layers. FEMS Microbiology Reviews, 20(1-2), 47-98. https://doi.org/10.1016/S0168-6445(97)00050-8
Bahl, Hubert ; Scholz, Holger ; Bayan, Nicolas ; Chami, Mohamed ; Leblon, Gérard ; Gulik-Krzywicki, Thaddée ; Shechter, Emanuel ; Fouet, Agnés ; Mesnage, Stéphane ; Tosi-Couture, Evelyne ; Gounon, Pierre ; Mock, Michèle ; De Macario, Everly Conway ; Macario, Alberto J.L. ; Fernández-Herrero, Luis A. ; Olabarría, Garbiñe ; Berenguer, José ; Blaser, Martin ; Kuen, Beatrix ; Lubitz, Werner ; Sára, Margit ; Pouwels, Peter H. ; Kolen, Carin P.A.M. ; Boot, Hein J. ; Palva, Airi ; Truppe, Michaela ; Howorka, Stephan ; Schroll, Gerhard ; Lechleitner, Sonja ; Resch, Stephanie. / IV. Molecular biology of S-layers. In: FEMS Microbiology Reviews. 1997 ; Vol. 20, No. 1-2. pp. 47-98.
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Bahl, H, Scholz, H, Bayan, N, Chami, M, Leblon, G, Gulik-Krzywicki, T, Shechter, E, Fouet, A, Mesnage, S, Tosi-Couture, E, Gounon, P, Mock, M, De Macario, EC, Macario, AJL, Fernández-Herrero, LA, Olabarría, G, Berenguer, J, Blaser, M, Kuen, B, Lubitz, W, Sára, M, Pouwels, PH, Kolen, CPAM, Boot, HJ, Palva, A, Truppe, M, Howorka, S, Schroll, G, Lechleitner, S & Resch, S 1997, 'IV. Molecular biology of S-layers', FEMS Microbiology Reviews, vol. 20, no. 1-2, pp. 47-98. https://doi.org/10.1016/S0168-6445(97)00050-8

IV. Molecular biology of S-layers. / Bahl, Hubert; Scholz, Holger; Bayan, Nicolas; Chami, Mohamed; Leblon, Gérard; Gulik-Krzywicki, Thaddée; Shechter, Emanuel; Fouet, Agnés; Mesnage, Stéphane; Tosi-Couture, Evelyne; Gounon, Pierre; Mock, Michèle; De Macario, Everly Conway; Macario, Alberto J.L.; Fernández-Herrero, Luis A.; Olabarría, Garbiñe; Berenguer, José; Blaser, Martin; Kuen, Beatrix; Lubitz, Werner; Sára, Margit; Pouwels, Peter H.; Kolen, Carin P.A.M.; Boot, Hein J.; Palva, Airi; Truppe, Michaela; Howorka, Stephan; Schroll, Gerhard; Lechleitner, Sonja; Resch, Stephanie.

In: FEMS Microbiology Reviews, Vol. 20, No. 1-2, 01.06.1997, p. 47-98.

Research output: Contribution to journalReview article

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T1 - IV. Molecular biology of S-layers

AU - Bahl, Hubert

AU - Scholz, Holger

AU - Bayan, Nicolas

AU - Chami, Mohamed

AU - Leblon, Gérard

AU - Gulik-Krzywicki, Thaddée

AU - Shechter, Emanuel

AU - Fouet, Agnés

AU - Mesnage, Stéphane

AU - Tosi-Couture, Evelyne

AU - Gounon, Pierre

AU - Mock, Michèle

AU - De Macario, Everly Conway

AU - Macario, Alberto J.L.

AU - Fernández-Herrero, Luis A.

AU - Olabarría, Garbiñe

AU - Berenguer, José

AU - Blaser, Martin

AU - Kuen, Beatrix

AU - Lubitz, Werner

AU - Sára, Margit

AU - Pouwels, Peter H.

AU - Kolen, Carin P.A.M.

AU - Boot, Hein J.

AU - Palva, Airi

AU - Truppe, Michaela

AU - Howorka, Stephan

AU - Schroll, Gerhard

AU - Lechleitner, Sonja

AU - Resch, Stephanie

PY - 1997/6/1

Y1 - 1997/6/1

N2 - In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is considerable variety in the S-layer composition, which was elucidated by sequence analysis of the corresponding genes. In Corynebacterium glutamicum one major cell wall protein is responsible for the formation of a highly ordered, hexagonal array. In contrast, two abundant surface proteins form the S-layer of Bacillus anthracis. Each protein possesses three S-layer homology motifs and one protein could be a virulence factor. The antigenic diversity and ABC transporters are important features, which have been studied in methanogenic archaea. The expression of the S-layer components is controlled by three genes in the case of Thermus thermophilus. One has repressor activity on the S-layer gene promoter, the second codes for the S-layer protein. The rearrangement by reciprocal recombination was investigated in Campylobacter fetus. 7-8 S-layer proteins with a high degree of homology at the 5' and 3' ends were found. Environmental changes influence the surface properties of Bacillus stearothermophilus. Depending on oxygen supply, this species produces different S-layer proteins. Finaly, the molecular bases for some applications are discussed. Recombinant S-layer fusion proteins have been designed for biotechnology.

AB - In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is considerable variety in the S-layer composition, which was elucidated by sequence analysis of the corresponding genes. In Corynebacterium glutamicum one major cell wall protein is responsible for the formation of a highly ordered, hexagonal array. In contrast, two abundant surface proteins form the S-layer of Bacillus anthracis. Each protein possesses three S-layer homology motifs and one protein could be a virulence factor. The antigenic diversity and ABC transporters are important features, which have been studied in methanogenic archaea. The expression of the S-layer components is controlled by three genes in the case of Thermus thermophilus. One has repressor activity on the S-layer gene promoter, the second codes for the S-layer protein. The rearrangement by reciprocal recombination was investigated in Campylobacter fetus. 7-8 S-layer proteins with a high degree of homology at the 5' and 3' ends were found. Environmental changes influence the surface properties of Bacillus stearothermophilus. Depending on oxygen supply, this species produces different S-layer proteins. Finaly, the molecular bases for some applications are discussed. Recombinant S-layer fusion proteins have been designed for biotechnology.

KW - ABC transporter

KW - Antigenic diversity

KW - Bacillus anthracis

KW - Bacillus stearothermophilus

KW - Corynebacterium glutamicum

KW - EAI protein

KW - Gene regulation

KW - Heterologous expression

KW - Lactobacilli

KW - Lactobacillus brevis

KW - Methanogenic archaea

KW - Methanosarcina mazei

KW - Oxygen stress

KW - PS2 protein

KW - S-layer

KW - S-layer gene

KW - S-layer protein

KW - S-layer structure-function relationship

KW - S-layer variation

KW - SLH motif

KW - Sap protein

KW - Sbs gene

KW - SbsA/SbsB fusion protein

KW - Secretion of β-lactamase

KW - Slp gene

KW - SlpA gene

KW - Thermus thermophilus

KW - cspB gene

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Bahl H, Scholz H, Bayan N, Chami M, Leblon G, Gulik-Krzywicki T et al. IV. Molecular biology of S-layers. FEMS Microbiology Reviews. 1997 Jun 1;20(1-2):47-98. https://doi.org/10.1016/S0168-6445(97)00050-8