Knockdown of dicer in MCF-7 human breast carcinoma cells results in G1 arrest and increased sensitivity to cisplatin

Ye Bu, Chunhua Lu, Chunjing Bian, Jinghua Wang, Jing Li, Bin Zhang, Zhenya Li, Gary Brewer, Robert Chunhua Zhao

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Aberrant expression of microRNAs (miRNAs) in various human cancers suggests a role for miRNAs in tumorigenesis. Dicer is an essential component of the miRNA machinery, which mediates the processing of miRNAs. However, little is known about the role of Dicer in tumor proliferation and drug resistance. In this study, we found that knockdown of Dicer by siRNA led to significant G1 arrest and increased sensitivity to the DNA damaging agent, cisplatin, in breast cancer cell line MCF-7. Moreover, we found down-regulation of miR-21, a well-recognized miRNA frequently involved in a wide variety of cancers and up-regulation of cell cycle-dependent kinase inhibitor (CKI) p21 and p27. These data demonstrate that knockdown of Dicer inhibits human breast carcinoma cell growth and suggests a promising combination of anti-Dicer strategy and traditional chemotherapy to improve cancer treatment efficiency.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalOncology reports
Volume21
Issue number1
DOIs
StatePublished - 2009

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Keywords

  • Cell cycle
  • Chemosensitivity
  • Cisplatin
  • Dicer
  • MicroRNA
  • SiRNA

Fingerprint

Dive into the research topics of 'Knockdown of dicer in MCF-7 human breast carcinoma cells results in G1 arrest and increased sensitivity to cisplatin'. Together they form a unique fingerprint.

Cite this