Laser-based mid-infrared reflectance imaging of biological tissues

  • Bujin Guo
  • , Y. Wang
  • , C. Peng
  • , H. L. Zhang
  • , G. P. Luo
  • , H. Q. Le
  • , C. Gmachl
  • , D. L. Sivco
  • , M. L. Peabody
  • , A. Y. Cho

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

Mid-infrared (MIR) (3-12 um) spectral imaging is a power analytical tool, but difficult in the back-reflectance mode for in-vivo diagnostics. Feasibility of MIR back-reflectance imaging is demonstrated using MIR semiconductor lasers. Transmittance through 500-μm thick films of water and blood showed a capability to resolve more than 6-OD signal dynamic range. Reflectance scanning imaging through a 150-μm thick film of blood showed negligible scattering effect, indicating the feasibility of optical coherent imaging. The result of coherent imaging of a plant leaf shows a MIR sub-surface image that would not be visible in white light. With two wavelengths, a similar result for a chicken skin subcutaneous tissue at different focal depths was obtained, showing blood vessels beneath a lipid layer. These results suggest that advanced multilaser wavelength systems in the fingerprint spectral region can be a useful tool for in-vivo spectral imaging in biomedical research and diagnostic applications.

Original languageAmerican English
Pages (from-to)208-219
Number of pages12
JournalOptics Express
Volume12
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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