Linoleic acid causes greater weight gain than saturated fat without hypothalamic inflammation in the male mouse

Kyle J. Mamounis, Ali Yasrebi, Troy Roepke

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A significant change in the Western diet, concurrent with the obesity epidemic, was a substitution of saturated fatty acids with polyunsaturated, specifically linoleic acid (LA). Despite increasing investigation on type as well as amount of fat, it is unclear which fatty acids are most obesogenic. The objective of this study was to determine the obesogenic potency of LA vs. saturated fatty acids and the involvement of hypothalamic inflammation. Forty-eight mice were divided into four groups: low-fat or three high-fat diets (HFDs, 45% kcals from fat) with LA comprising 1%, 15% and 22.5% of kilocalories, the balance being saturated fatty acids. Over 12 weeks, bodyweight, body composition, food intake, calorimetry, and glycemia assays were performed. Arcuate nucleus and blood were collected for mRNA and protein analysis. All HFD-fed mice were heavier and less glucose tolerant than control. The diet with 22.5% LA caused greater bodyweight gain, decreased activity, and insulin resistance compared to control and 1% LA. All HFDs elevated leptin and decreased ghrelin in plasma. Neuropeptides gene expression was higher in 22.5% HFD. The inflammatory gene Ikk was suppressed in 1% and 22.5% LA. No consistent pattern of inflammatory gene expression was observed, with suppression and augmentation of genes by one or all of the HFDs relative to control. These data indicate that, in male mice, LA induces obesity and insulin resistance and reduces activity more than saturated fat, supporting the hypothesis that increased LA intake may be a contributor to the obesity epidemic.

Original languageEnglish (US)
Pages (from-to)122-131
Number of pages10
JournalJournal of Nutritional Biochemistry
Volume40
DOIs
StatePublished - Feb 1 2017

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Linoleic Acid
Weight Gain
Fats
Inflammation
Fatty Acids
Nutrition
Obesity
Gene expression
Insulin Resistance
Genes
Insulin
Gene Expression
Arcuate Nucleus of Hypothalamus
Calorimetry
Ghrelin
High Fat Diet
Leptin
Body Composition
Neuropeptides
Assays

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Nutrition and Dietetics
  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

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title = "Linoleic acid causes greater weight gain than saturated fat without hypothalamic inflammation in the male mouse",
abstract = "A significant change in the Western diet, concurrent with the obesity epidemic, was a substitution of saturated fatty acids with polyunsaturated, specifically linoleic acid (LA). Despite increasing investigation on type as well as amount of fat, it is unclear which fatty acids are most obesogenic. The objective of this study was to determine the obesogenic potency of LA vs. saturated fatty acids and the involvement of hypothalamic inflammation. Forty-eight mice were divided into four groups: low-fat or three high-fat diets (HFDs, 45{\%} kcals from fat) with LA comprising 1{\%}, 15{\%} and 22.5{\%} of kilocalories, the balance being saturated fatty acids. Over 12 weeks, bodyweight, body composition, food intake, calorimetry, and glycemia assays were performed. Arcuate nucleus and blood were collected for mRNA and protein analysis. All HFD-fed mice were heavier and less glucose tolerant than control. The diet with 22.5{\%} LA caused greater bodyweight gain, decreased activity, and insulin resistance compared to control and 1{\%} LA. All HFDs elevated leptin and decreased ghrelin in plasma. Neuropeptides gene expression was higher in 22.5{\%} HFD. The inflammatory gene Ikk was suppressed in 1{\%} and 22.5{\%} LA. No consistent pattern of inflammatory gene expression was observed, with suppression and augmentation of genes by one or all of the HFDs relative to control. These data indicate that, in male mice, LA induces obesity and insulin resistance and reduces activity more than saturated fat, supporting the hypothesis that increased LA intake may be a contributor to the obesity epidemic.",
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Linoleic acid causes greater weight gain than saturated fat without hypothalamic inflammation in the male mouse. / Mamounis, Kyle J.; Yasrebi, Ali; Roepke, Troy.

In: Journal of Nutritional Biochemistry, Vol. 40, 01.02.2017, p. 122-131.

Research output: Contribution to journalArticle

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