Live cell labeling of glial progenitor cells using targeted quantum dots

Nidhi Sabharwal, Eric C. Holland, Maribel Vazquez

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

This study describes the development of targeted quantum dots (T-QDs) as biomarkers for the labeling of glial progenitor cells (GPCs) that over express platelet derived growth factor (PDGF) and its receptor PDGFR (GPC PDGF). PDGFR plays a critical role in glioma development and growth, and is also known to affect multiple biological processes such as cell migration and embryonic development. T-QDs were developed using streptavidin-conjugated quantum dots (S-QDs) with biotinylated antibodies and utilized to label the intracellular and extracellular domains of live, cultured GPCPDGF cells via lipofection with cationic liposomes. Confocal studies illustrate successful intracellular and extracellular targeted labeling within live cells that does not appear to impact upstream PDGFR dynamics during real-time signaling events. Further, T-QDs were nontoxic to GPCPDGF cells, and did not alter cell viability or proliferation over the course of 6 days. These results raise new applications for T-QDs as ultra sensitive agents for imaging and tracking of protein populations within live cells, which that will enable future mechanistic study of oncogenic signaling events in real-time.

Original languageEnglish (US)
Pages (from-to)1967-1973
Number of pages7
JournalAnnals of Biomedical Engineering
Volume37
Issue number10
DOIs
StatePublished - Oct 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering

Keywords

  • Cationic liposome
  • Glial progenitor cells
  • Live cell labeling
  • Platelet derived growth factor
  • Quantum dots

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