Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse

Ju Chen; Changshun Shao; Virginie Lazar; Carolyn H. Srivastava; Wei Hua Lee; Jay A. Tischfield

doi:10.1002/(SICI)1097-4644(19970301)64:3

Localization of group IIc low molecular weight phospholipase A₂ mRNA to meiotic cells in the mouse

Ju Chen
, Changshun Shao
, Virginie Lazar
, Carolyn H. Srivastava
, Wei Hua Lee
, Jay A. Tischfield

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

We use in situ hybridization to demonstrate that the testicular expression of a novel, mouse, low molecular weight phospholipase A₂ (PLA₂ Group IIc) mRNA is specific to cells undergoing meiosis. A complete cDNA (1421 bp) encoding the mouse Pla2g2c gene was generated with reverse transcription-PCR (RT-PCR) and 5' and 3' RACE (rapid amplification of cDNA ends) RT-PCR, and this nucleotide sequence was determined. Northern blots of RNA from different tissues revealed a single 1.6 kb transcript only in testis. In situ hybridization indicated that this mouse gene is transcribed mainly in pachytene spermatocytes, secondary spermatocytes, and round spermatids. Expression of the gene is seen in all stages of the seminiferous epithelium, especially in stages VI-VII.

Original language	American English
Pages (from-to)	369-375
Number of pages	7
Journal	Journal of Cellular Biochemistry
Volume	64
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1097-4644(19970301)64:3
State	Published - Mar 1 1997
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Keywords

cDNA
in situ hybridization
meiosis
mouse
phospholipase A
pla2g2c
spermatocytes
testis

Access to Document

10.1002/(SICI)1097-4644(19970301)64:3

Cite this

@article{bdc290e89c294ed496373fba8c0017b3,

title = "Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse",

abstract = "We use in situ hybridization to demonstrate that the testicular expression of a novel, mouse, low molecular weight phospholipase A2 (PLA2 Group IIc) mRNA is specific to cells undergoing meiosis. A complete cDNA (1421 bp) encoding the mouse Pla2g2c gene was generated with reverse transcription-PCR (RT-PCR) and 5' and 3' RACE (rapid amplification of cDNA ends) RT-PCR, and this nucleotide sequence was determined. Northern blots of RNA from different tissues revealed a single 1.6 kb transcript only in testis. In situ hybridization indicated that this mouse gene is transcribed mainly in pachytene spermatocytes, secondary spermatocytes, and round spermatids. Expression of the gene is seen in all stages of the seminiferous epithelium, especially in stages VI-VII.",

keywords = "cDNA, in situ hybridization, meiosis, mouse, phospholipase A, pla2g2c, spermatocytes, testis",

author = "Ju Chen and Changshun Shao and Virginie Lazar and Srivastava, \{Carolyn H.\} and Lee, \{Wei Hua\} and Tischfield, \{Jay A.\}",

year = "1997",

month = mar,

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doi = "10.1002/(SICI)1097-4644(19970301)64:3",

language = "American English",

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TY - JOUR

T1 - Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse

AU - Chen, Ju

AU - Shao, Changshun

AU - Lazar, Virginie

AU - Srivastava, Carolyn H.

AU - Lee, Wei Hua

AU - Tischfield, Jay A.

PY - 1997/3/1

Y1 - 1997/3/1

N2 - We use in situ hybridization to demonstrate that the testicular expression of a novel, mouse, low molecular weight phospholipase A2 (PLA2 Group IIc) mRNA is specific to cells undergoing meiosis. A complete cDNA (1421 bp) encoding the mouse Pla2g2c gene was generated with reverse transcription-PCR (RT-PCR) and 5' and 3' RACE (rapid amplification of cDNA ends) RT-PCR, and this nucleotide sequence was determined. Northern blots of RNA from different tissues revealed a single 1.6 kb transcript only in testis. In situ hybridization indicated that this mouse gene is transcribed mainly in pachytene spermatocytes, secondary spermatocytes, and round spermatids. Expression of the gene is seen in all stages of the seminiferous epithelium, especially in stages VI-VII.

AB - We use in situ hybridization to demonstrate that the testicular expression of a novel, mouse, low molecular weight phospholipase A2 (PLA2 Group IIc) mRNA is specific to cells undergoing meiosis. A complete cDNA (1421 bp) encoding the mouse Pla2g2c gene was generated with reverse transcription-PCR (RT-PCR) and 5' and 3' RACE (rapid amplification of cDNA ends) RT-PCR, and this nucleotide sequence was determined. Northern blots of RNA from different tissues revealed a single 1.6 kb transcript only in testis. In situ hybridization indicated that this mouse gene is transcribed mainly in pachytene spermatocytes, secondary spermatocytes, and round spermatids. Expression of the gene is seen in all stages of the seminiferous epithelium, especially in stages VI-VII.

KW - cDNA

KW - in situ hybridization

KW - meiosis

KW - mouse

KW - phospholipase A

KW - pla2g2c

KW - spermatocytes

KW - testis

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