@inbook{4e90c83cd1044cd1b73973af03fb5a1e,
title = "Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence",
abstract = "Visualization of in vivo protein levels and localization is essential to analysis and elucidation of Hippo signaling mechanisms and its roles in diverse tissues. This is best done by imaging proteins using fluorescent labels. Fluorescent labeling of a protein can be achieved by direct conjugation to an intrinsically fluorescent protein, like GFP, or by use of antibodies conjugated to fluorescent dyes. Immunofluorescence imaging in Drosophila typically begins with dissection and fixation of a sample tissue, followed by a series of washes and incubations with primary antibodies, directed against proteins of interest, and dye-labeled secondary antibodies, directed against the primary antibodies. This may be followed by fluorescent dyes that label cellular components, such as DNA-labeling dyes to mark nuclei. After staining and washing is completed, samples are placed in a mounting media, transferred to a microscope slide, and imaged on a confocal microscope.",
keywords = "Antibody, Confocal, GFP, Hippo, Immunofluorescence, Yorkie",
author = "Cordelia Rauskolb and Irvine, {Kenneth D.}",
note = "Funding Information: Research in the Irvine laboratory is supported by NIH grants GM78620 and GM121537. Funding Information: by NIH grants Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
doi = "10.1007/978-1-4939-8910-2_5",
language = "American English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "61--73",
booktitle = "Methods in Molecular Biology",
}