Matrix assembly, cell polarization, and cell survival: analysis of peri-implantation development with cultured embryonic stem cells.

Shaohua Li; Peter D. Yurchenco

Matrix assembly, cell polarization, and cell survival: analysis of peri-implantation development with cultured embryonic stem cells.

Shaohua Li, Peter D. Yurchenco

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A variety of mutations, including those affecting laminin expression and basement membrane, cause early embryonic lethality in the peri-implantation period. However, low cell numbers and inaccessibility of these small embryos make it difficult to study the molecular mechanisms that underlie these defects. Embryoid bodies cultured as suspended spherical cell aggregates derived from normal and defective embryonic stem cells provide a tractable experimental system with which the early developmental processes can be recapitulated under defined conditions. Thus, endoderm formation and maturation, basement membrane assembly and its signaling consequences, epiblast polarization, apoptosis, and cavitation can be studied using a combination of genetic, biochemical, cell, and molecular biology approaches.

Original language	American English
Pages (from-to)	113-125
Number of pages	13
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	329
State	Published - 2006

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

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AB - A variety of mutations, including those affecting laminin expression and basement membrane, cause early embryonic lethality in the peri-implantation period. However, low cell numbers and inaccessibility of these small embryos make it difficult to study the molecular mechanisms that underlie these defects. Embryoid bodies cultured as suspended spherical cell aggregates derived from normal and defective embryonic stem cells provide a tractable experimental system with which the early developmental processes can be recapitulated under defined conditions. Thus, endoderm formation and maturation, basement membrane assembly and its signaling consequences, epiblast polarization, apoptosis, and cavitation can be studied using a combination of genetic, biochemical, cell, and molecular biology approaches.

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Matrix assembly, cell polarization, and cell survival: analysis of peri-implantation development with cultured embryonic stem cells.

Abstract

ASJC Scopus subject areas

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