Metabolic response of vetiver grass (Chrysopogon zizanioides) to acid mine drainage

Jeffrey D. Kiiskila, Kefeng Li, Dibyendu Sarkar, Rupali Datta

Research output: Contribution to journalArticle

Abstract

Acid mine drainage (AMD) is a sulfuric discharge containing metals and particulates that can spread to nearby water sources, imposing toxicity and physical stress to living things. We have shown that vetiver grass (Chrysopogon zizanioides) is capable of tolerating and treating AMD-impacted water from the abandoned Tab-Simco mining site from southern Illinois, though little is known about its tolerance mechanisms. We conducted metabolomic analyses of vetiver shoots and roots after relatively short- and long-term periods of exposure to Tab-Simco AMD. The metabolic shift of vetiver shoots was dramatic with longer-term AMD exposure, including upregulation of amino acid and glutathione metabolism, cellular respiration and photosynthesis pathways, with downregulation of phosphorylated metabolites. Meanwhile, the roots demonstrated drastic downregulation of phospholipids and phosphorylated metabolites, cellular respiration, glyoxylate metabolism, and amino acid metabolism. Vetiver accumulated ornithine and oxaloacetate in the shoots, which could function for nitrogen storage and various intracellular functions, respectively. Organic acids and glutathione were secreted from the roots for rhizospheric metal-chelation, whereas phosphorylated metabolites were recycled for phosphorus. These findings reveal AMD-induced metabolic shifts in vetiver grass, which are seemingly unique in comparison to independent abiotic stresses reported previously.

Original languageEnglish (US)
Article number124961
JournalChemosphere
Volume240
DOIs
StatePublished - Feb 2020

Fingerprint

Vetiveria
acid mine drainage
Drainage
grass
Metabolites
Acids
Metabolism
metabolite
shoot
Cell Respiration
metabolism
Glutathione
Amino acids
respiration
amino acid
Metals
Down-Regulation
Amino Acids
Oxaloacetic Acid
chelation

All Science Journal Classification (ASJC) codes

  • Pollution
  • Chemistry(all)
  • Health, Toxicology and Mutagenesis
  • Environmental Engineering
  • Environmental Chemistry

Cite this

Kiiskila, Jeffrey D. ; Li, Kefeng ; Sarkar, Dibyendu ; Datta, Rupali. / Metabolic response of vetiver grass (Chrysopogon zizanioides) to acid mine drainage. In: Chemosphere. 2020 ; Vol. 240.
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Metabolic response of vetiver grass (Chrysopogon zizanioides) to acid mine drainage. / Kiiskila, Jeffrey D.; Li, Kefeng; Sarkar, Dibyendu; Datta, Rupali.

In: Chemosphere, Vol. 240, 124961, 02.2020.

Research output: Contribution to journalArticle

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