MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction

TY - JOUR

T1 - MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction

AU - Li, Jingyuan

AU - Li, Jing

AU - Liu, Xiaojun

AU - Qin, Shanshan

AU - Guan, Yanzhong

AU - Liu, Yuwei

AU - Cheng, Yunhui

AU - Chen, Xiuwen

AU - Li, Wen

AU - Wang, Shenming

AU - Xiong, Ming

AU - Kuzhikandathil, Eldo V.

AU - Ye, Jiang Hong

AU - Zhang, Chunxiang

PY - 2013/9

Y1 - 2013/9

N2 - Alcohol addiction is a major social and health concern. Here, we determined the expression profile of microRNAs (miRNAs) in the nucleus accumbens (NAc) of rats treated with alcohol. The results suggest that multiple miRNAs were aberrantly expressed in rat NAc after alcohol injection. Among them, miR-382 was down-regulated in alcohol-treated rats. In both cultured neuronal cells in vitro and in the NAc in vivo, we identified that the dopamine receptor D1 (Drd1) is a direct target gene of miR-382. Via this target gene, miR-382 strongly modulated the expression of DeltaFosB. Moreover, overexpression of miR-382 significantly attenuated alcohol-induced up-regulation of DRD1 and DeltaFosB, decreased voluntary intake of and preference for alcohol and inhibited the DRD1-induced action potential responses. The results indicated that miRNAs are involved in and may represent novel therapeutic targets for alcoholism.

AB - Alcohol addiction is a major social and health concern. Here, we determined the expression profile of microRNAs (miRNAs) in the nucleus accumbens (NAc) of rats treated with alcohol. The results suggest that multiple miRNAs were aberrantly expressed in rat NAc after alcohol injection. Among them, miR-382 was down-regulated in alcohol-treated rats. In both cultured neuronal cells in vitro and in the NAc in vivo, we identified that the dopamine receptor D1 (Drd1) is a direct target gene of miR-382. Via this target gene, miR-382 strongly modulated the expression of DeltaFosB. Moreover, overexpression of miR-382 significantly attenuated alcohol-induced up-regulation of DRD1 and DeltaFosB, decreased voluntary intake of and preference for alcohol and inhibited the DRD1-induced action potential responses. The results indicated that miRNAs are involved in and may represent novel therapeutic targets for alcoholism.

KW - Alcohol addiction

KW - DeltaFosB

KW - Dopamine receptor D1

KW - MiR-382

KW - MicroRNAs

UR - http://www.scopus.com/inward/record.url?scp=84883360733&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883360733&partnerID=8YFLogxK

U2 - https://doi.org/10.1002/emmm.201201900

DO - https://doi.org/10.1002/emmm.201201900

M3 - Article

C2 - 23873704

SN - 1757-4676

VL - 5

SP - 1402

EP - 1414

JO - EMBO Molecular Medicine

JF - EMBO Molecular Medicine

IS - 9

ER -