MicroRNA-repressed mRNAs contain 40S but not 60S components

Bingbing Wang, Adrienne Yanez, Carl D. Novina

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


MicroRNAs (miRNAs) are small noncoding RNAs that may target more than one-third of human genes, yet the mechanisms used by miRNAs to repress translation of target mRNAs are obscure. Using a recently described cell-free assay of miRNA function, we observe that miRNA-targeted mRNAs are enriched for 40S but not 60S ribosome components. Additionally, toeprinting analysis of miRNA-targeted mRNAs demonstrates that ≈18 nt 3′ relative to the initiating AUG are protected, consistent with 40S ribosome subunits positioned at the AUG codon. Our results suggest that miRNAs repress translation initiation by preventing 60S subunit joining to miRNA-targeted mRNAs.

Original languageEnglish (US)
Pages (from-to)5343-5348
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number14
StatePublished - Apr 8 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


  • Argonaute
  • Ribosome
  • Translation


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