Missense mutations in the unfoldase ClpC1 of the caseinolytic protease complex are associated with pyrazinamide resistance in Mycobacterium tuberculosis

Michelle Yee, Pooja Gopal, Thomas Dick

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Previously, we showed that mutations in Mycobacterium tuberculosis panD, involved in coenzyme A biosynthesis, cause resistance against pyrazinoic acid, the bioactive component of the prodrug pyrazinamide. To identify additional resistance mechanisms, we isolated mutants resistant against pyrazinoic acid and subjected panD wild-type strains to whole-genome sequencing. Eight of the nine resistant strains harbored missense mutations in the unfoldase ClpC1 associated with the caseinolytic protease complex.

Original languageEnglish (US)
Article numbere02342
JournalAntimicrobial agents and chemotherapy
Volume61
Issue number2
DOIs
StatePublished - Feb 1 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Infectious Diseases
  • Pharmacology

Keywords

  • Caseinolytic protease
  • ClpC1
  • Mycobacterium tuberculosis
  • Pyrazinamide
  • Resistance

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