Molecular regulation of human interleukin 4 and T cell subset development

C. Hu, M. Li-Weber, P. Salgame

Research output: Contribution to journalArticle

Abstract

The Th1 and Th2 subsets of T cells are defined on the basis of their cytokine profiles. On activation, Thl cells secrete Interferon (IFN)-y, Interleukin (IL)-2, and Tumor Necrosis factor (TNF)-ot, whereas Th2 cells produce IL-4, IL-5, and IL-10. The two subsets arise from a common post-thymic precursor ThO-type cell expressing multiple cytokines and this process is influenced by a number of factors. The clearest examples of such factors are IL-12 and IFN-y, which promote differentiation of Th precursors into Th1 cells, and IL-4 which has the reciprocal activity, inducing differentiation to Th2 cells. The present work explores the hypothesis that ability of IFN-γ to promote T cell differentiation to Th1-type results from transcriptional downregulation of the IL-4 gene. IFN-γ pretreatment of precursor ThO cells, prior to stimulation, resulted in a decrease in transcription of the IL-4 gene and also altered the pattern of nuclear binding proteins for the IL-4 regulatory element, PRE-1. Experiments are ongoing to determine if these alterations in IL-4 gene transcription promote ThO cells to develop into stable Thl-type cells.

Original languageEnglish (US)
Pages (from-to)A1051
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Biotechnology

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