Mouse Models of Thyroid Neoplasia: Insights into Thyroid Pathophysiology

TY - CHAP

T1 - Mouse Models of Thyroid Neoplasia

T2 - Insights into Thyroid Pathophysiology

AU - Antonello, Z. A.

AU - Nucera, C.

N1 - Publisher Copyright: © 2014 Elsevier Inc. All rights reserved.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Thyroid carcinoma is the most common endocrine maLignancy, and papillary thyroid carcinoma (PTC) accounts for over 90% of all thyroid maLignancies. The incidence of thyroid cancer, mainly PTC, has been rising rapidly in recent decades worldwide. One major challenge of basic and medical research is to create in vitro and in vivo models to recapitulate in a controlled and investigable manner different aspects of tumor and cancer disease, such as in situ growth, local invasion, and distant colonization (metastasis). From this perspective, simpLicity of the model is a major advantage. However, the finis ultimus of biomedical research is to define effective pharmacological treatments for diseases. In this other outlook, conservation from model to human and model complexity are desired.We will discuss in vivo experimental approaches estabLished in mice to study thyroid cancers, giving particular attention to the advantages and disadvantages of the different models, pathophysiological conservation with humans, and practical biomedical questions that each of them helps to tackle.We will first present subcutaneous and orthotopic xenograft models and describe their properties. Then, we will describe imaging techniques, which are fundamental for analysis, and also discuss the cell Lines on which they are based. Secondly, we will cover genetically modified models, describing transgenic models and knockout/knockin models grouped by the type of thyroid cancer that they reproduce.

AB - Thyroid carcinoma is the most common endocrine maLignancy, and papillary thyroid carcinoma (PTC) accounts for over 90% of all thyroid maLignancies. The incidence of thyroid cancer, mainly PTC, has been rising rapidly in recent decades worldwide. One major challenge of basic and medical research is to create in vitro and in vivo models to recapitulate in a controlled and investigable manner different aspects of tumor and cancer disease, such as in situ growth, local invasion, and distant colonization (metastasis). From this perspective, simpLicity of the model is a major advantage. However, the finis ultimus of biomedical research is to define effective pharmacological treatments for diseases. In this other outlook, conservation from model to human and model complexity are desired.We will discuss in vivo experimental approaches estabLished in mice to study thyroid cancers, giving particular attention to the advantages and disadvantages of the different models, pathophysiological conservation with humans, and practical biomedical questions that each of them helps to tackle.We will first present subcutaneous and orthotopic xenograft models and describe their properties. Then, we will describe imaging techniques, which are fundamental for analysis, and also discuss the cell Lines on which they are based. Secondly, we will cover genetically modified models, describing transgenic models and knockout/knockin models grouped by the type of thyroid cancer that they reproduce.

UR - https://www.scopus.com/pages/publications/85043279244

UR - https://www.scopus.com/pages/publications/85043279244#tab=citedBy

U2 - 10.1016/B978-0-12-386456-7.03704-7

DO - 10.1016/B978-0-12-386456-7.03704-7

M3 - Chapter

SN - 9780123864574

SP - 1206

EP - 1222

BT - Pathobiology of Human Disease

PB - Elsevier Inc.

ER -