Multiple insertions and tandem repeats of origin-minus simian virus 40 DNA in transformed rat and mouse cells

TY - JOUR

T1 - Multiple insertions and tandem repeats of origin-minus simian virus 40 DNA in transformed rat and mouse cells

AU - Blanck, G.

AU - Li, D.

AU - Pomert, E.

AU - Pollack, R.

AU - Chen, S.

PY - 1988

Y1 - 1988

N2 - Stable simian virus 40 (SV40) transformation requires integration and expression of the early region of the SV40 genome. We have examined the amount and state of integrated viral DNA of SV40-transformed NIH 3T3 mouse and F2408 rat fibroblast lines generated by transfection with either wild-type or origin-defective SV40 DNA. A functional SV40 replication origin was not required for multiple inserts and partial-repeat structures to form in NIH 3T3 mouse transformants. In contrast, partial repeats in F2408 rat transformants were rare when the SV40 replication origin was intact and not detected at all when it was defective.

AB - Stable simian virus 40 (SV40) transformation requires integration and expression of the early region of the SV40 genome. We have examined the amount and state of integrated viral DNA of SV40-transformed NIH 3T3 mouse and F2408 rat fibroblast lines generated by transfection with either wild-type or origin-defective SV40 DNA. A functional SV40 replication origin was not required for multiple inserts and partial-repeat structures to form in NIH 3T3 mouse transformants. In contrast, partial repeats in F2408 rat transformants were rare when the SV40 replication origin was intact and not detected at all when it was defective.

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U2 - https://doi.org/10.1128/jvi.62.5.1520-1523.1988

DO - https://doi.org/10.1128/jvi.62.5.1520-1523.1988

M3 - Article

C2 - 2833605

SN - 0022-538X

VL - 62

SP - 1520

EP - 1523

JO - Journal of virology

JF - Journal of virology

IS - 5

ER -