Multiple roles of the RNA polymerase β subunit flap domain in σ54-dependent transcription

Siva R. Wigneshweraraj, Konstantin Kuznedelov, Konstantin Severinov, Martin Buck

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recent determinations of the structures of the bacterial RNA polymerase (RNAP) and promoter complex thereof establish that RNAP functions as a complex molecular machine that contains distinct structural modules that undergo major conformational changes during transcription. However, the contribution of the RNAP structural modules to transcription remains poorly understood. The bacterial core RNAP (α2ββ′ω; E) associates with a sigma (σ) subunit to form the holoenzyme (Eσ). A mutation removing the β subunit flap domain renders the Escherichia coli σ70 RNAP holoenzyme unable to recognize promoters. σ54 is the major variant σ subunit that utilizes enhancer-dependent promoters. Here, we determined the effects of β flap removal on σ54-dependent transcription. Our analysis shows that the role of the β flap in σ54-dependent and σ70-dependent transcription is different. Removal of the β flap does not prevent the recognition of σ54-dependent promoters, but causes multiple defects in σ54-dependent transcription. Most importantly, the β flap appears to orchestrate the proper formation of the Eσ54 regulatory center at the start site proximal promoter element where activator binds and DNA melting originates.

Original languageEnglish (US)
Pages (from-to)3455-3465
Number of pages11
JournalJournal of Biological Chemistry
Volume278
Issue number5
DOIs
StatePublished - Jan 31 2003

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DNA-Directed RNA Polymerases
Transcription
Bacterial RNA
Holoenzymes
Nucleic Acid Denaturation
Sigma Factor
Escherichia coli
Melting
Defects
Mutation
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Wigneshweraraj, Siva R. ; Kuznedelov, Konstantin ; Severinov, Konstantin ; Buck, Martin. / Multiple roles of the RNA polymerase β subunit flap domain in σ54-dependent transcription. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 5. pp. 3455-3465.
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Multiple roles of the RNA polymerase β subunit flap domain in σ54-dependent transcription. / Wigneshweraraj, Siva R.; Kuznedelov, Konstantin; Severinov, Konstantin; Buck, Martin.

In: Journal of Biological Chemistry, Vol. 278, No. 5, 31.01.2003, p. 3455-3465.

Research output: Contribution to journalArticle

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