TY - JOUR
T1 - Natural cytotoxic cells against solid tumors in mice. IV. Natural cytotoxic (NC) cells are not activated natural killer (NK) cells
AU - Lattime, Edmund C.
AU - Pecoraro, Gene
AU - Stutman, Osias
PY - 1982/10/15
Y1 - 1982/10/15
N2 - Natural cell‐mediated cytotoxicity (NCMC), measured against a variety of tumors, is mediated by at least two sub‐populations of effector cells: natural cytotoxic (NC) and natural killer (NK). The studies described in this report show that target lysis by NC cells requires a prolonged (18‐ to 24 h) assay period, whereas NC‐susceptible targets can be lysed in short‐term (4 h) [3H]‐proline assays using allo‐sensitized CTL or mitogen‐activated cytotoxic populations. NC cells are not “activated” during the long‐term assay as indicated by: (1) demonstrating that lysis of NC‐susceptible targets in long‐term (20 h) 51Cr assays is still the function of a Qa‐5 effector cell (NC) and (2) the fact that preincubation of the NC effector cell with susceptible targets for 18 h did not result in the activation of an NK‐like population (kinetics of target lysis were comparable to those noted with fresh NC cell preparations). We show that NC cell activity is preserved in both the beige mutant and the PL/J mouse strains, both of which exhibit low NK cell activity, even in long‐term assays. These combined studies support the view that NC and NK cell activities are the function of distinct cell types and not the property of a single cell class under different states of activation.
AB - Natural cell‐mediated cytotoxicity (NCMC), measured against a variety of tumors, is mediated by at least two sub‐populations of effector cells: natural cytotoxic (NC) and natural killer (NK). The studies described in this report show that target lysis by NC cells requires a prolonged (18‐ to 24 h) assay period, whereas NC‐susceptible targets can be lysed in short‐term (4 h) [3H]‐proline assays using allo‐sensitized CTL or mitogen‐activated cytotoxic populations. NC cells are not “activated” during the long‐term assay as indicated by: (1) demonstrating that lysis of NC‐susceptible targets in long‐term (20 h) 51Cr assays is still the function of a Qa‐5 effector cell (NC) and (2) the fact that preincubation of the NC effector cell with susceptible targets for 18 h did not result in the activation of an NK‐like population (kinetics of target lysis were comparable to those noted with fresh NC cell preparations). We show that NC cell activity is preserved in both the beige mutant and the PL/J mouse strains, both of which exhibit low NK cell activity, even in long‐term assays. These combined studies support the view that NC and NK cell activities are the function of distinct cell types and not the property of a single cell class under different states of activation.
UR - https://www.scopus.com/pages/publications/0019918810
UR - https://www.scopus.com/pages/publications/0019918810#tab=citedBy
U2 - 10.1002/ijc.2910300414
DO - 10.1002/ijc.2910300414
M3 - Article
C2 - 7141742
SN - 0020-7136
VL - 30
SP - 471
EP - 477
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 4
ER -