Abstract
We have generated a murine embryonic stem cell line constitutively expressing L1 at all stages of neural differentiation to investigate the effects of L1 overexpression on stem cell proliferation, migration, differentiation, cell death, and ability to influence drug-induced rotation behavior in an animal model of Huntington's disease. L1-transfected cells showed decreased cell proliferation in vitro, enhanced neuronal differentiation in vitro and in vivo, and decreased astrocytic differentiation in vivo without influencing cell death compared with nontransfected cells. L1 overexpression also resulted in an increased yield of GABAergic neurons and enhanced migration of embryonic stem cell-derived neural precursor cells into the lesioned striatum. Mice grafted with L1-transfected cells showed recovery in rotation behavior 1 and 4 weeks, but not 8 weeks, after transplantation compared with mice that had received nontransfected cells, thus demonstrating for the first time that a recognition molecule is capable of improving functional recovery during the initial phase in a syngeneic transplantation paradigm.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11532-11539 |
| Number of pages | 8 |
| Journal | Journal of Neuroscience |
| Volume | 26 |
| Issue number | 45 |
| DOIs | |
| State | Published - Nov 8 2006 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
Keywords
- Embryonic stem cell
- Functional recovery
- Neural cell adhesion molecule L1
- Neuronal differentiation
- Quinolinic acid
- Striatum
Cite this
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Neural cell adhesion molecule L1-transfected embryonic stem cells promote functional recovery after excitotoxic lesion of the mouse striatum. / Bernreuther, Christian; Dihné, Marcel; Johann, Verena; Schiefer, Johannes; Cui, Yifang; Hargus, Gunnar; Schmid, Janinne Sylvie; Xu, Jinchong; Kosinski, Christoph M.; Camartin, Melitta.
In: Journal of Neuroscience, Vol. 26, No. 45, 08.11.2006, p. 11532-11539.Research output: Contribution to journal › Article
TY - JOUR
T1 - Neural cell adhesion molecule L1-transfected embryonic stem cells promote functional recovery after excitotoxic lesion of the mouse striatum
AU - Bernreuther, Christian
AU - Dihné, Marcel
AU - Johann, Verena
AU - Schiefer, Johannes
AU - Cui, Yifang
AU - Hargus, Gunnar
AU - Schmid, Janinne Sylvie
AU - Xu, Jinchong
AU - Kosinski, Christoph M.
AU - Camartin, Melitta
PY - 2006/11/8
Y1 - 2006/11/8
N2 - We have generated a murine embryonic stem cell line constitutively expressing L1 at all stages of neural differentiation to investigate the effects of L1 overexpression on stem cell proliferation, migration, differentiation, cell death, and ability to influence drug-induced rotation behavior in an animal model of Huntington's disease. L1-transfected cells showed decreased cell proliferation in vitro, enhanced neuronal differentiation in vitro and in vivo, and decreased astrocytic differentiation in vivo without influencing cell death compared with nontransfected cells. L1 overexpression also resulted in an increased yield of GABAergic neurons and enhanced migration of embryonic stem cell-derived neural precursor cells into the lesioned striatum. Mice grafted with L1-transfected cells showed recovery in rotation behavior 1 and 4 weeks, but not 8 weeks, after transplantation compared with mice that had received nontransfected cells, thus demonstrating for the first time that a recognition molecule is capable of improving functional recovery during the initial phase in a syngeneic transplantation paradigm.
AB - We have generated a murine embryonic stem cell line constitutively expressing L1 at all stages of neural differentiation to investigate the effects of L1 overexpression on stem cell proliferation, migration, differentiation, cell death, and ability to influence drug-induced rotation behavior in an animal model of Huntington's disease. L1-transfected cells showed decreased cell proliferation in vitro, enhanced neuronal differentiation in vitro and in vivo, and decreased astrocytic differentiation in vivo without influencing cell death compared with nontransfected cells. L1 overexpression also resulted in an increased yield of GABAergic neurons and enhanced migration of embryonic stem cell-derived neural precursor cells into the lesioned striatum. Mice grafted with L1-transfected cells showed recovery in rotation behavior 1 and 4 weeks, but not 8 weeks, after transplantation compared with mice that had received nontransfected cells, thus demonstrating for the first time that a recognition molecule is capable of improving functional recovery during the initial phase in a syngeneic transplantation paradigm.
KW - Embryonic stem cell
KW - Functional recovery
KW - Neural cell adhesion molecule L1
KW - Neuronal differentiation
KW - Quinolinic acid
KW - Striatum
UR - http://www.scopus.com/inward/record.url?scp=33751087210&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751087210&partnerID=8YFLogxK
U2 - https://doi.org/10.1523/JNEUROSCI.2688-06.2006
DO - https://doi.org/10.1523/JNEUROSCI.2688-06.2006
M3 - Article
C2 - 17093074
VL - 26
SP - 11532
EP - 11539
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 45
ER -