Next-generation ammonia pretreatment enhances cellulosic biofuel production

Leonardo Da Costa Sousa, Mingjie Jin, Shishir P.S. Chundawat, Vijay Bokade, Xiaoyu Tang, Ali Azarpira, Fachuang Lu, Utku Avci, James Humpula, Nirmal Uppugundla, Christa Gunawan, Sivakumar Pattathil, Albert M. Cheh, Ninad Kothari, Rajeev Kumar, John Ralph, Michael G. Hahn, Charles E. Wyman, Seema Singh, Blake A. SimmonsBruce E. Dale, Venkatesh Balan

Research output: Contribution to journalArticlepeer-review

127 Scopus citations


A new liquid ammonia pretreatment methodology called Extractive Ammonia (EA) was developed to simultaneously convert native crystalline cellulose Iβ (CI) to a highly digestible cellulose IIII (CIII) allomorph and selectively extract up to ∼45% of the lignin from lignocellulosic biomass with near-quantitative retention of all polysaccharides. EA pretreated corn stover yielded a higher fermentable sugar yield compared to the older Ammonia Fiber Expansion (AFEX) process while using 60% lower enzyme loading. The EA process preserves extracted lignin functionalities, offering the potential to co-produce lignin-derived fuels and chemicals in the biorefinery. The single-stage EA fractionation process achieves high biofuel yields (18.2 kg ethanol per 100 kg untreated corn stover, dry weight basis), comparable to those achieved using ionic liquid pretreatments. The EA process achieves these ethanol yields at industrially-relevant conditions using low enzyme loading (7.5 mg protein per g glucan) and high solids loading (8% glucan, w/v).

Original languageEnglish (US)
Pages (from-to)1215-1223
Number of pages9
JournalEnergy and Environmental Science
Issue number4
StatePublished - Apr 2016

All Science Journal Classification (ASJC) codes

  • Pollution
  • Nuclear Energy and Engineering
  • Renewable Energy, Sustainability and the Environment
  • Environmental Chemistry


Dive into the research topics of 'Next-generation ammonia pretreatment enhances cellulosic biofuel production'. Together they form a unique fingerprint.

Cite this