Nondefective spleen necrosis virus-derived vectors define the upper size limit for packaging reticuloendotheliosis viruses

Celine Gelinas, H. M. Temin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We constructed a nondefective retrovirus vector based on spleen necrosis virus (SNV), a replication-completent reticuloendotheliosis virus. We introduced different DNA sequences into this vector and studied the ability of the resulting viruses to replicate in chicken embryo fibroblasts. The replication efficiency of SNV-derived viruses decreased with increasing virus size. Viruses larger than 9.4 kilobases (kb) were rapidly overgrown by replication-competent deletion mutants. The size restriction for the efficient replication of nondefective SNV-derived viruses prevented the production of viruses larger than 10.0 kb. Analysis of the kinetics of virus particle release indicated that the size restriction occurred during virus encapsidation.

Original languageEnglish (US)
Pages (from-to)9211-9215
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number23
DOIs
StatePublished - Jan 1 1986

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Trager duck spleen necrosis virus
Reticuloendotheliosis virus
Product Packaging
Viruses
Virus Release
Retroviridae
Virus Replication
Virion
Chickens
Embryonic Structures
Fibroblasts

All Science Journal Classification (ASJC) codes

  • General

Cite this

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