Novel findings regarding Glut-4 expression in adipose tissue and muscle in horses - A preliminary report

Helio C. Manso Filho, Kenneth H. McKeever, Mary E. Gordon, Helena Emilia C. Costa, Malcolm Watford

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

One of the hallmarks of insulin resistance is a reduction in glucose transporter-4 (Glut-4) expression in adipose tissue but not in skeletal muscle. However, while Glut-4 has been demonstrated in skeletal and cardiac muscles in horses it has not been demonstrated in adipose tissue. The initial objectives of the present study were: (1) to test the hypothesis that Glut-4 expression would vary between selected key skeletal muscles; (2) to test the hypothesis that it would also vary between representative adipose tissue depots, and (3) to see whether expression would be greater in adipose tissue compared to muscle. Glut-4 expression was determined by Western blot using samples obtained from post mortem biopsies obtained from four muscles (gluteus medius, semitendinosus, heart, and diaphragm), and four adipose tissues (subcutaneous, retroperitoneal, mesenteric, and omental) in three horses. There were no differences (P > 0.05) in Glut-4 protein expression between the muscles sampled. Likewise there were no differences (P > 0.05) in Glut-4 protein expression between fat depots. There was a significant difference (P = 0.03) when pooled means for Glut-4 expression in muscle (58.8 ± 2.5 densitometry units) were compared with adipose tissue (115.8 ± 15.7). This difference in Glut-4 expression in these two tissues with distinctly different metabolic reasons for taking up glucose may warrant further investigation to see if there are more pronounced differences in Glut-4 expression in muscle and adipose tissue in various populations of horses.

Original languageEnglish (US)
Pages (from-to)565-569
Number of pages5
JournalVeterinary Journal
Volume174
Issue number3
DOIs
StatePublished - Nov 2007

All Science Journal Classification (ASJC) codes

  • veterinary(all)
  • Animal Science and Zoology

Keywords

  • Body composition
  • Equine
  • Glucose metabolism
  • Insulin sensitivity

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