Novel Fri1‐like viruses infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12— characterization, comparative genomic analysis, and host‐recognition strategy

Anastasia V. Popova, Daria G. Lavysh, Evgeniy I. Klimuk, Mikhail V. Edelstein, Alexander G. Bogun, Mikhail M. Shneider, Artemiy E. Goncharov, Sergey V. Leonov, Konstantin Severinov

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Acinetobacter baumannii is a gram‐negative, non‐fermenting aerobic bacterium which is often associated with hospital‐acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two novel viruses, vB_AbaP_AS11 and vB_AbaP_AS12, specifically infecting and lysing distinct multidrug‐resistant clinical A. baumannii strains with K19 and K27 capsular polysaccharide structures, respectively. Both phages demonstrate rapid adsorption, short latent periods, and high burst sizes in one‐step growth experiments. The AS11 and AS12 linear double‐stranded DNA genomes of 41,642 base pairs (bp) and 41,402 bp share 86.3% nucleotide sequence identity with the most variable regions falling in host receptor–recognition genes. These genes encode tail spikes possessing depolymerizing activities towards corresponding capsular polysaccharides which are the primary bacterial receptors. We described AS11 and AS12 genome organization and discuss the possible regulation of transcription. The overall genomic architecture and gene homology analyses showed that the phages are new representatives of the recently designated Fri1virus genus of the Autographivirinae subfamily within the Podoviridae family.

Original languageEnglish (US)
Article number188
JournalViruses
Volume9
Issue number7
DOIs
StatePublished - Jul 17 2017

Fingerprint

Acinetobacter
Acinetobacter baumannii
Viruses
Base Pairing
Bacteriophages
Polysaccharides
Podoviridae
Genome
Genes
Aerobic Bacteria
Biofilms
Microbial Drug Resistance
Adsorption
DNA
Growth
Infection

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

Keywords

  • Acinetobacter baumannii
  • Bacteriophage
  • Capsule types
  • Fri1virus
  • Podoviridae
  • RNA polymerase
  • Tail spike

Cite this

Popova, Anastasia V. ; Lavysh, Daria G. ; Klimuk, Evgeniy I. ; Edelstein, Mikhail V. ; Bogun, Alexander G. ; Shneider, Mikhail M. ; Goncharov, Artemiy E. ; Leonov, Sergey V. ; Severinov, Konstantin. / Novel Fri1‐like viruses infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12— characterization, comparative genomic analysis, and host‐recognition strategy. In: Viruses. 2017 ; Vol. 9, No. 7.
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Novel Fri1‐like viruses infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12— characterization, comparative genomic analysis, and host‐recognition strategy. / Popova, Anastasia V.; Lavysh, Daria G.; Klimuk, Evgeniy I.; Edelstein, Mikhail V.; Bogun, Alexander G.; Shneider, Mikhail M.; Goncharov, Artemiy E.; Leonov, Sergey V.; Severinov, Konstantin.

In: Viruses, Vol. 9, No. 7, 188, 17.07.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Novel Fri1‐like viruses infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12— characterization, comparative genomic analysis, and host‐recognition strategy

AU - Popova, Anastasia V.

AU - Lavysh, Daria G.

AU - Klimuk, Evgeniy I.

AU - Edelstein, Mikhail V.

AU - Bogun, Alexander G.

AU - Shneider, Mikhail M.

AU - Goncharov, Artemiy E.

AU - Leonov, Sergey V.

AU - Severinov, Konstantin

PY - 2017/7/17

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N2 - Acinetobacter baumannii is a gram‐negative, non‐fermenting aerobic bacterium which is often associated with hospital‐acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two novel viruses, vB_AbaP_AS11 and vB_AbaP_AS12, specifically infecting and lysing distinct multidrug‐resistant clinical A. baumannii strains with K19 and K27 capsular polysaccharide structures, respectively. Both phages demonstrate rapid adsorption, short latent periods, and high burst sizes in one‐step growth experiments. The AS11 and AS12 linear double‐stranded DNA genomes of 41,642 base pairs (bp) and 41,402 bp share 86.3% nucleotide sequence identity with the most variable regions falling in host receptor–recognition genes. These genes encode tail spikes possessing depolymerizing activities towards corresponding capsular polysaccharides which are the primary bacterial receptors. We described AS11 and AS12 genome organization and discuss the possible regulation of transcription. The overall genomic architecture and gene homology analyses showed that the phages are new representatives of the recently designated Fri1virus genus of the Autographivirinae subfamily within the Podoviridae family.

AB - Acinetobacter baumannii is a gram‐negative, non‐fermenting aerobic bacterium which is often associated with hospital‐acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two novel viruses, vB_AbaP_AS11 and vB_AbaP_AS12, specifically infecting and lysing distinct multidrug‐resistant clinical A. baumannii strains with K19 and K27 capsular polysaccharide structures, respectively. Both phages demonstrate rapid adsorption, short latent periods, and high burst sizes in one‐step growth experiments. The AS11 and AS12 linear double‐stranded DNA genomes of 41,642 base pairs (bp) and 41,402 bp share 86.3% nucleotide sequence identity with the most variable regions falling in host receptor–recognition genes. These genes encode tail spikes possessing depolymerizing activities towards corresponding capsular polysaccharides which are the primary bacterial receptors. We described AS11 and AS12 genome organization and discuss the possible regulation of transcription. The overall genomic architecture and gene homology analyses showed that the phages are new representatives of the recently designated Fri1virus genus of the Autographivirinae subfamily within the Podoviridae family.

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KW - Bacteriophage

KW - Capsule types

KW - Fri1virus

KW - Podoviridae

KW - RNA polymerase

KW - Tail spike

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