Nuclear magnetic resonance studies on wheat germ agglutinin-monomeric amino sugar interactions

Frank Jordan, Hanae Bahr, John Patrick, Peter W.K. Woo

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The interaction of several N-acetyl-d-glucosamine analogs and of sialyl lactose with the lectin wheat germ agglutinin was studied by nuclear magnetic resonance. N-2H3-acetyl-d-gluocosamine was synthesized and found to displace the N-acetyl methyl signal toward its free chemical shift in N-acetylglucosamine and N-acetylneuraminic acid demonstrating common binding sites for the latter two compounds. The N-acetyl methyl signal of the α-methylglucoside of N-acetylglucosamine could be titrated but a 3-deoxy analog could not, the latter exhibiting very weak binding and demonstrating the importance of the 3-OH group in the binding process. Sialyl lactose (an N-acetylneuraminic acid analog) was rather tightly bound to the lectin. N-F3-acetyl-d-glucosamine was synthesized and its binding to the lectin was studied at pH 4, 4.5, 5.1 by 19F NMR. The two anomers were found to bind with nearly equal Kd′s but exhibited a pH and anomer dependent Δ (total bound chemical shift). The -CF3 analog was found to bind considerably stronger to the lectin than the -CH3 compound. The clear resolution of the α and β anomers of this molecule make it a very useful probe of the lectin binding site.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Mar 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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