(-)-Oleocanthal and (-)-oleocanthal-rich olive oils induce lysosomal membrane permeabilization in cancer cells

Limor Goren, George Zhang, Susmita Kaushik, Paul Breslin, Yi Chieh Nancy Du, David A. Foster

Research output: Contribution to journalArticle

Abstract

(-)-Oleocanthal (oleocanthal) is a phenolic compound found in varying concentrations in extra virgin olive oil oleocanthal has been shown to be active physiologically, benefiting several diseased states by conferring anti-inflammatory and neuroprotective benefits. Recently, we and other groups have demonstrated its specific and selective toxicity toward cancer cells; however, the mechanism leading to cancer cell death is still disputed. The current study demonstrates that oleocanthal, as well as naturally oleocanthal-rich extra virgin olive oils, induced damage to cancer cells' lysosomes leading to cellular toxicity in vitro and in vivo. Lysosomal membrane permeabilization following oleocanthal treatment in various cell lines was assayed via three complementary methods. Additionally, we found oleocanthal treatment reduced tumor burden and extended lifespan of mice engineered to develop pancreatic neuroendocrine tumors. Finally, following-up on numerous correlative studies demonstrating consumption of olive oil reduces cancer incidence and morbidity, we observed that extra virgin olive oils naturally rich in oleocanthal sharply reduced cancer cell viability and induced lysosomal membrane permeabilization while oleocanthal-poor oils did not. Our results are especially encouraging since tumor cells often have larger and more numerous lysosomes, making them especially vulnerable to lysosomotropic agents such as oleocanthal.

Original languageEnglish (US)
Article numbere0216024
JournalPLoS ONE
Volume14
Issue number8
DOIs
StatePublished - Jan 1 2019

Fingerprint

olive oil
Cells
Membranes
Neoplasms
extra-virgin olive oil
Tumors
lysosomes
Lysosomes
neoplasms
Toxicity
oleocanthal
neoplasm cells
Olive Oil
Neuroendocrine Tumors
Cell death
Tumor Burden
cell viability
morbidity
cytotoxicity
cell death

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • General
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Goren, Limor ; Zhang, George ; Kaushik, Susmita ; Breslin, Paul ; Du, Yi Chieh Nancy ; Foster, David A. / (-)-Oleocanthal and (-)-oleocanthal-rich olive oils induce lysosomal membrane permeabilization in cancer cells. In: PLoS ONE. 2019 ; Vol. 14, No. 8.
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(-)-Oleocanthal and (-)-oleocanthal-rich olive oils induce lysosomal membrane permeabilization in cancer cells. / Goren, Limor; Zhang, George; Kaushik, Susmita; Breslin, Paul; Du, Yi Chieh Nancy; Foster, David A.

In: PLoS ONE, Vol. 14, No. 8, e0216024, 01.01.2019.

Research output: Contribution to journalArticle

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